Polytetrafluoroethylene added to acrylic resin: Surface properties and Candida albicans adherence

Abstract

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Purpose To evaluate the surface properties and Candida albicans adherence on two acrylic resins, one polymerized by long cycle and the other by short polymerization cycle, with added 2% PTFE (wt/wt) (experimental groups) or not added (control groups) Methods The specimens were divided into four groups (n= 10 per group) (1) acrylic resin polymerized by long cycle, (2) acrylic resin polymerized by long cycle with added 2% PTFE, (3) acrylic resin polymerized by short cycle, and (4) acrylic resin polymerized by short cycle with added 2% PTFE After finishmg and polishing, all specimens were assessed for their surface roughness (mu m), contact angle (degrees) and surface free energy (erg/cm (2)) Saliva-coated specimens were submitted to the adherence assay with C albicans (ATCC 90028) Adhered cells were detached from the acrylic resin surface by ultrasonic waves at 7 watts for 30 seconds m phosphate buffered saline solution (PBS) This cell solution was serially diluted m PBS and plated on Sabouraud agar The results were expressed m colony forming units per surface area (CFU/mm(2)) The data was analyzed by two-way ANOVA The significance limit was set at 5% Results No statistical differences were found for roughness, contact angle and surface free energy There was a reduction trend in C albicans adherence in PTFE added to resins, but it was not statistically significant (Am J Dent 2010,23 201-204)234201204Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAPESP [2006/03090 7