Preparation of a C-18 stationary phase immobilized by gamma radiation for use in high performance liquid chromatography

Abstract

A liquid stationary phase, poly(methyloctadecylsiloxane), was immobilized on a silica support, in the proportion 50:50 m/m, by gamma radiation doses of 20 to 90 kGy, with the purpose of obtaining a highly stable chromatographic stationary phase. This stationary phase exhibited higher efficiency and shorter analysis times than similar columns prepared with commercial C-18 packings.21162447245