Behaviour Of Albino And Melanic Variants Of Biomphalaria Glabrata Say, 1818 (mollusca: Planorbidae) Following Infection By Schistosoma Mansoni Sambon, 1907 [comportamento De Biomphalaria Glabrata Variantes Albina E Melânica Frente à Infecção Pelo Schistosoma Mansoni]

Abstract

The behaviour of the albino and melanic variants of Biomphalaria glabrata of Belo Horizonte (MG. Brazil) was studied comparatively, in terms of their respective susceptibilities to infection by Schistosoma mansoni of the same origin, through observation of the elimination of cercariae for a three-month period and the calculation of mortality and infection rates, in control and in infected snails. The number of amoebocytes, granulocytes and hyalinocytes in the circulating hemolymph during different periods of infection was analyzed. The evolution of the infection in the tissues was observed by means of histological cross-sections. The melanic variant showed greater susceptibility to infection and a higher mortality rate. The albino variant showed a higher number of circulating amoebocytes, both granulocytes and hyalinocytes. A higher number of degenerated sporocysts were seen in the histological cross-sections of the albino variant. The results suggest that the melanic variant of B. glabrata was more susceptible to infection by S. mansoni than was the albino variant.691217222Abdul-Salam, J.M., Michelson, E.H., Biomphalaria glabrata amebocytes: Effect of Schistosoma mansoni infection on in vitro phagocytosis (1980) J. Invertebr. Pathol., 35 (3), pp. 241-248Balan, D.S.L., Magalhães, L.A., Piedrabuena, A.E., Aspectos imunológicos e parasitológicos em Biomphalaria tenagophila infectadas por Schistosoma mansoni e outros Digenea (1993) Rev. Saúde Pública, 27 (6), pp. 21-429Barbosa, L., Silva, L.M., Coelho, P.M.Z., Santos, S.R., Fortes-Dias, C.L., Primary culture of the region of the amebocyte-producing organ of the snail Biomphalaria glabrata, the intermediate host of Schistosoma mansoni (2006) Mem. Inst. Oswaldo Cruz, 101 (6), pp. 639-643Barbosa, L., Caldeira, R.L., Carvalho, O.S., Vidigal, T.H.D.A., Jannotti-Passos, L.K., Coelho, P.Z., Resistance to Schistosoma mansoni by transplantation of APO Biomphalaria tenagophila (2006) Parasite Immunol., 28 (5), pp. 209-212Bayne, C.J., Loker, E.S., Yui, M.A., Interactions between the plasma proteins of Biomphalaria glabrata (Gastropoda) and the sporocyst tegument of Schistosoma mansoni (Trematode) (1986) Parasitol., 92 (3), pp. 653-664Boswell, C.A., Bayne, C.J., Lectin-dependent cell-mediated cytotoxicity in an invertebrate model: Con A does not act as bridge (1986) Immunology., 57 (2), pp. 261-264Carton, Y., Nappi, A.J., Poirie, M., Genetics of anti-parasite resistance in invertebrates (2005) Dev. Comp. Immunol., 29 (1), pp. 9-32Castillo, M.G., Yoshino, T.P., Carbohydrate inhibition of Biomphalaria glabrata embryonic (Bge) cell adhesion to primary sporocysts to Schistosoma mansoni (2002) Parasitology, 125 (6), pp. 513-525. , (Pt 6)Connors, V.A., Yoshino, T.P., In vitro effect of larval Schistosoma mansoni excretory-secretory products on phagocytosis-stimulated superoxide production in hemocytes from Biomphalaria glabrata (1990) J. Parasitol., 76 (6), pp. 895-902Granath Jr., W.O., Yoshino, T.P., Schistosoma mansoni: Passive transfer of resistance by serum in the vector snail, Biomphalaria glabrata (1984) Exp. Parasitol., 58 (2), pp. 188-193Guaraldo, A.M.A., Magalhães, L.A., Rangel, H.A., Pareja, G., Evolução dos esporocistos de Schistosoma mansoni Sambon, 1907, em Biomphalaria glabrata (Say, 1818) e Biomphalaria tenagophila (D'Orbigny, 1835) (1981) Rev. Saúde Pública, 15 (4), pp. 436-448Hahn, U.K., Bender, R.C., Bayne, C.J., Killing of Schistosoma mansoni sporocysts by hemocytes from resistant Biomphalaria glabrata: Role of reactive oxygen species (2001) J. Parasitol., 87 (2), pp. 292-299Lie, K.J., Jeong, K.H., Heyneman, D., Tissues reactions induced by Schistosoma mansoni in Biomphalaria glabrata (1980) Ann. Trop. Med. Parasitol., 74 (2), pp. 157-166Loker, E.S., Bayne, C.J., In vitro encounters between Schistosoma mansoni primary sporocysts and hemolymph components of susceptible and resistant strains of Biomphalaria glabrata (1982) Am. J. Trop. Med. Hyg., 31 (5), pp. 999-1005Loker, E.S., Adema, C.M., Zhang, S.M., Kepler, T.B., Invertebrate immune systems - not homogeneous, not simple, not well understood (2004) Immunol. Rev., 198 (1), pp. 10-24Martins-Souza, R.L., Pereira, C.A.J., Martins Filho, A.O., Coelho, P.M.Z., Corrêa Jr., A., Negrão-Corrêa, D., Differential lectin labelling of circulanting hemocytes from Biomphalaria glabrata and Biomphalaria tenagophila resistant or suceptible to Schistosoma mansoni infection (2006) Mem. Inst. Oswaldo Cruz., 101 (SUPPL. I), pp. 185-192Matricon-Gondran, M., Joke, A., Benex, J., Responses of the amebocytes producing organ (APO) in Biomphalaria glabrata susceptible or resistant to trematode invasion: An ultrastructural study (1986) Dev. Comp. Immunol., 10 (4), p. 636Newton, W.L., The inheritance of susceptibility to infection with Schistosoma mansoni in Australorbis glabratus (1953) Exp. Parasitol, 2 (3), pp. 242-257Paraense, W.L., Corrêa, L.R., Variation in susceptibility of populations of Australorbis glabratus to a strain of Schistosoma mansoni (1963) Rev. Inst. Med. Trop. São Paulo, 5 (1), pp. 15-22Richards, C.S., Merritt, J.W., Genetic factors in the susceptibility for juvenile Biomphalaria glabrata to Schistosoma mansoni infection (1972) Am. J. Trop. Med. Hyg., 21 (4), pp. 425-434Richards, C.S., Susceptibility of adult Biomphalaria glabrata to Schistosoma mansoni infection (1973) Am. J. Trop. Med. Hyg., 22 (6), pp. 748-756Richards, C.S., Influence of snail age one genetic variations in susceptibility of Biomphalaria glabrata for infections with Schistosoma mansoni (1984) Malacologia, 25 (2), pp. 493-502Santana, J.V., (1990) Mecanismo de defesa em Biomphalaria tenagophila e Biomphalaria glabrata frente ao Schistosoma mansoni, , Campinas: Universidade Estadual de Campinas. (Tese de doutoradoSeta, L., Magalhães, L.A., Carvalho, J.F., Comportamento dos amebócitos circulantes de moluscos planorbídeos frente ao parasitismo por larvas de Schistosoma mansoni, à inoculação de tinta Nanquin ou à fratura da concha (1996) Rev. Saúde Publ., 30 (4), pp. 332-340Sminia, T., van Knaap, W.P.W., Cells and molecules in molluscan immunology (1987) Dev. Comp. Immunol., 11 (1), pp. 17-28Stumpf, J.L., Gilbertson, D.E., Hemocytes of Biomphalaria glabrata: Factor affecting variability (1978) J. Invert. Pathol., 32 (2), pp. 177-181Tripp, M.R., Molluscan immunity (1974) Ann. Acad. N.Y. Sci., 234 (1), pp. 23-27Wright, C.A., Ross, G.C., Electrophoretic studies of blood and egg proteins in Australorbis glabratus (Gastropoda, Planorbidae) (1963) Ann. Trop. Med. Parasit., 57 (1), pp. 47-5