The objective of this work was to evaluate a set of microsatellite markers for varietal identification and characterization of the most widespread potato cultivars in Brazil. The DNA from 14 potato cultivars was genotyped using microsatellite markers and the alleles were scored in silver-stained polyacrylamide gel. Twenty-four microsatellite markers were evaluated, and only one locus was monomorphic. Based on band patterns, a set of two microsatellites that were able to identify and differentiate all examined cultivars was obtained.451110113Collares, E.A.S., Choer, E., Pereira, A.S., Characterization of potato genotypes using molecular markers (2004) Pesquisa Agropecuária Brasileira, 39, pp. 871-878Creste, S., Tulmann, A., Figueira, A., Detection of Single Sequence Repeat Polymorphism in denaturating polyacrylamide sequencing gels by silver staining (2001) Plant Molecular Biology Reporter, 19, pp. 299-306Demecke, T., Kawchuk, L.M., Linch, D.R., Identification of potato cultivars and clonal variants by Random Amplified Polymorphic DNA analysis (1993) American Potato Journal, 70, pp. 561-570Douches, D.S., Ludlan, K., Eletrophoretic characterization of North American potato cultivars (1991) American Potato Journal, 68, pp. 767-780Feingold, S., Lloyd, J., Norero, N., Bonierbale, M., Lorenzen, J., Mapping and characterization of new EST-derived microsatellites for potato (Solanum tuberosum L.) (2005) Theoretical and Applied Genetics, 111, pp. 456-466Ghislain, M., Spooner, D.M., Rodríguez, F., Villamón, F., Núnez, J., Vásquez, C., Waugh, R., Bonierbale, M., Selection of highly informative and user-friendly microsatellites (SSRs) for genotyping of cultivated potato (2004) Theoretical and Applied Genetics, 108, pp. 881-890Isenegger, D.A., Taylor, P.W.J., Ford, R., Franz, P., Mcgregor, G.R., Hutchinson, J.F., DNA fingerprinting and genetic relationships of potato cultivars (Solanum tuberosum L.) commercially grown in Australia (2001) Australian Journal of Agricultural Research, 52, pp. 911-918Kim, J.H., Juong, H., Kim, H.Y., Lim, Y.P., Estimation of genetic variation and relationship in potato (Solanum tuberosum L.) cultivars using AFLP markers (1998) American Journal of Potato Research, 75, pp. 107-112Mathias, M.R., Sagrado, B.D., Kalazich, J.B., Use of SSR markers to identify potato germplasm in INIA Chile breeding program (2007) Agricultura Técnica, 67, pp. 3-15McGregor, C.E., Lambert, C.A., Greyling, M.M., Louw, J.H., Warnich, L., A comparative assessment of DNA fingerprinting techniques (RAPD, ISSR AFLP and SSR) in tetraploid potato (Solanum tuberosum L.) germplasm (2000) Euphytica, 113, pp. 135-144Moisan-Thiery, M., Marhadour, S., Kerlan, M.C., Dessenne, N., Perramant, M., Gokelalre, T., Le Hingrat, Y., Potato cultivar identification using simple sequence repeats markers (SSR) (2005) Potato Research, 48, pp. 191-200Norero, N., Malleville, J., Huarte, M., Feingold, S., Cost efficient potato (Solanum tuberosum L.) cultivar identification by microsatellite amplification (2002) Potato Research, 45, pp. 131-138Reid, A., Kerr, E.M., A rapid simple sequence repeat (SSR)-based identification method for potato cultivars (2007) Plant Genetic Resources: Characterization and Utilization, 5, pp. 7-13Tessier, C., David, J., This, P., Boursiquot, J.M., Charrier, A., Optimization of the choice of molecular markers for varietal identification in Vitis vinifera L (1999) Theoretical and Applied Genetics, 98, pp. 171-177Wulff, E.G., Torres, S., Gonzales, E.V., Protocol for DNA extraction from potato tubers (2002) Plant Molecular Biology Reporter, 20, pp. 187a-1187
