Susceptibility Of Cell Lines To Avian Viruses

Abstract

The susceptibility of the five cell lines - IB-RS-2, RK-13, Vero, BHK-21, CER - to reovirus S1133 and infectious bursal disease virus (IBDV vaccine GBV-8 strain) was studied to better define satisfactory and sensitive cell culture systems. Cultures were compared for presence of CPE, virus titers and detection of viral RNA. CPE and viral RNA were detected in CER and BHK-21 cells after reovirus inoculation and in RK-13 cell line after IBDV inoculation and with high virus titers. Virus replication by production of low virus titers occurred in IB-RS-2 and Vero cells with reovirus and in BHK-21 cell line with IBDV.304373376Barta, V., Springer, W.T., Millar, D.L., A comparison of avian and mammalian cell cultures for the propagation of avian reovirus WVU 2937 (1984) Avian Dis., 28, pp. 216-223Cowen, B.S., Braune, M.O., The propagation of avian viruses in a continuous cell line (QT35) of Japanese quail origin (1988) Avian Dis., 32, pp. 282-297Fernandes, M.J.B., Simoni, I.C., Caracterização de linhagens celulares: II- Suscetibilidade aos virus da doença de Aujeszky e da Febre aftosa (1995) Arq. Inst. Biol., 62, pp. 65-72Giambrone, J.J., Microculture neutralization test for serodiagnosis of three avian viral infections (1980) Avian Dis., 24, pp. 2284-2287Guneratne, J.R.M., Jones, R.C., Georgiou, K., Some observations on the isolation and cultivation of avian reoviruses (1982) Avian Pathol., 11, pp. 453-462Herrings, A.J., Inglis, N.F., Ojeh, C.K., Snodgrass, D.R., Menzies, J.D., Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels (1982) J. Clin. Microbiol., 16, pp. 473-477Kibenge, F.S.B., Dhillon, A.S., Russell, R.G., Growth of serotypes I and II and variant strains of infectious bursal disease virus in Vero cells (1988) Avian Dis., 32, pp. 298-303Kibenge, F.S.B., Mckenna, P.K., Isolation and propagation of infectious bursal disease virus using the ovine kidney continuous cell line (1992) Avian Dis., 36, pp. 256-261Jackwood, D.H., Saif, Y.M., Hughes, J.H., Replication of infectious bursal disease virus in continuous cell lines (1987) Avian Dis., 31, pp. 370-375Jones, R.C., Different sensitivity of Vero cells from two sources to avian reoviruses (1990) Res. Vet. Science, 48, pp. 379-380Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685Lukert, P.D., Leonard, J., Davis, R.B., Infectious bursal disease virus: Antigen production and immunity (1975) Am. J. Vet. Res., 36, pp. 539-540Lukert, P.D., Saif, Y.M., Infectious bursal disease (1991) Disease of Poultry, 9th Ed., pp. 648-663. , B.W. Calnek, H.J. Barnes, C.W. Beard, W.M. Reid and H.W. Yoder, Jr. eds. Iowa State University Press, Ames, IowaPereira, H.G., Azeredo, R.S., Leite, J.P.G., Electrophoretic study of the genome of human rotaviruses from Rio de Janeiro, São Paulo and Pará, Brazil (1983) J. Hyg. Camb., 90, pp. 117-125Petek, M., D'Aprile, P.N., Cancellotti, F., Biological and phisico-chemical properties of the infectious bursal disease virus (IBDV) (1973) Avian Pathol., 2, pp. 135-152Reed, L.J., Mucnch, H.A., A simple method of estimating fifty percent endpoints (1938) Am. J. Hyg., 27, pp. 493-497Robertson, M.D., Wilcox, G.E., Avian reovirus (1986) Vet. Bull., 56, pp. 155-174Rosenberg, J.K., Olson, N.O., Reovirus Infections (1991) Disease of Poultry, pp. 639-647. , Calnck, H.J.Barnes, C.W.Beard, W.M.Yoder, H.W., 9th edit. Iowa State Univ. Press, AmesWilcox, G.E., Robertson, M.D., Lines, A.D., Adaptation and characteristics of replication of a strain reovirus in Vero cells (1985) Avian Pathol., 14, pp. 321-32