Preliminary Characterization And Grouping Of Candida Species By Numerical Analysis Of Protein Profiles Obtained By Polyacrylamide Gel Electrophoresis

Abstract

Whole-cell proteins from isolates of five Candida species (Candida albicans, Candida krusei, Candida parapsilosis, Candida tropicalis and Candida guilliermondii) were separated by SDS-PAGE and the profiles obtained were converted into a binary data matrix that produced a cophenetic correlation phenogram. The analysis of the phenogram allowed detection of the cophenetic correlation levels existing among these species.1612729Greenspan, D., Treatment of oral candidiasis in HIV infection (1984) Oral Surg, 78, pp. 211-215Lynch, D.P., Oral candidiasis History, classification, and clinical presentation (1994) Oral Surg, 78, pp. 189-193Asakura, K., Iwaguchi, S.I., Homma, M., Sukai, T., Higashide, K., Tanaka, K., Electrophoretic karyotypes of clinically isolated yeasts Candida albicans and C glabrata (1991) J Gen Microbiol, 137, pp. 2531-2538Monod, M., Porchet, F., Baudraz-Rosselet, Frenk, E., The identification of pathogenic yeast strains by electrophoretic analysis of their chromosomes (1990) J Med Microbiol, 32, pp. 123-129Scherer, S., Stevens, D.A., Application of DNA typing methods to epidemiology and taxonomy of Candida species (1987) J Clin Microbiol, 25, pp. 675-679Lehmann, P.F., Kemker, B.J., Hsiao, C.B., Dev, S., Isoenzyme biotypes of Candida species (1989) J Clin Microbiol, 27, pp. 2514-2521Casanova, M., Chaffin, W.L., Cell wall glycoproteins of Candida albicans as released by different methods (1991) J Gen Microbiol, 137, pp. 1045-1051Shen, H.D., Choo, K.B., Tsai, W.C., Jen, T.M., Yeh, J.Y., Han, S.H., Differential identification of Candida species and other yeasts by analysis of [35S] - Metionine labeled polypeptide profiles (1988) Anal Biochem, 175, pp. 548-555Bruneau, S., Guinet, R., Rapid identification of medically important yeast by electrophoretic protein patterns (1989) FEMS Microbiol Letters, 58, pp. 329-334Maiden, M.F.J., Tanner, A., Identification of oral yeasts by polyacrylamide gel electrophoresis (1991) Oral Microbiol Immun, 6, pp. 187-190Tanner, A.C.R., Listgarten, M.A., Ebersole, J.L., Strzemkpo, M.N., Bacteroides forsythus sp nov , a slow-growing, fusiform Bacteroides sp from the human oral cavity (1986) Int J Syst Bact, 36, pp. 213-221Costas, M., Holmes, B., Wood, A.C., On, S.L.W., Numerical analysis of electrophoretic patterns of Providencia rettgeri from human faeces, urine and other specimens (1989) J Appl Bact, 67, pp. 441-452Holmes, B., Costas, M., Sloss, L.L., Numerical analysis of SDS-PAGE protein patterns of Serratia marcescens: A comparison with other typing methods (1990) Epidemiol Infect, 105, pp. 107-117Vancanneyt, M., Lerberge, E.V., Berny, J.F., Hennebert, G.L., Kersters, K., The application of whole-cell protein electrophoresis for the classification and identification of basidiomycetous yeast species (1992) Antonie Van Leeuwenhoek, 61, pp. 69-78Vancanneyt, M., Pot, B., Hennebert, G., Kersters, K., Differentiation of yeast species based on electrophoretic whole-cell protein patterns (1991) Syst Appl Microbiol, 14, pp. 23-32Höfling, J.F., Rosa, E.A.R., Rochelle, S.L.A., Spolidorio, D.M.P., Moreira, D., Numerical analysis variations of SDS-PAGE protein patterns using different culture media for the cultivation of oral yeast cells (1998) Rev Microbiol, 29, pp. 79-84Kersters, K., Numerical methods in the classification of bacteria by protein eletrophoresis (1985) Computer-assisted Bacterial Systematics, pp. 337-368. , Goodfellow M, Jones D, Priesr FG (Eds.) London, Academic PressWoontner, M., Jaehning, J.A., Accurate initiation by RNA polymerase II in a whole cell extract from Saccharomyces cerevisiae (1990) J Biol Chem, 265, pp. 8979-8982Bradford, M.M., A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal Biochem, 72, pp. 248-254Ames, G.F.L., Resolution of bacterial proteins by polyacrylamide gel electrophoresis on slabs (1974) J Biol Chem, 249, pp. 634-644Laemmli, U.K., Cleavage of strutural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685Costas, M., Sloss, L.L., Owen, R.J., Gaston, M.A., Evaluation of numerical analysis of SDS-PAGE of protein patterns for typing Enterobacter cloacae (1989) Epidemiol Infect, 103, pp. 265-274Sneath, P.H.A., Sokal, R.Q., (1973) Numerical Taxonomy, , San Francisco, FreemanKersters, K., De Ley, J., Identification and grouping of bacteria by numerical analysis of their electrophoretic proteins patterns (1975) J Gen Microbiol, 87, pp. 333-342Tanner, A.C.R., Characterization of Wolinnella spp, Campylobacter concisus, Bacterroides gracilis, and Eikenella corrodens by polyacrylamide gel electrophoresis (1986) J Clin Microbiol, 24, pp. 562-565Sneath, P.H.A., Johnson, R., The influence on numerical taxonomy similarities of errors in microbial testes (1972) J Gen Microbiol, 72, pp. 248-255Shechter, Y., Landau, J.W., Dabrowa, N., Comparative electrophoresis and numeral taxonomy of some Candida species (1972) Mycologia, 64, pp. 841-853Barns, S.M., Lane, D.J., Sogin, M.L., Bibeau, C., Weisburg, W.G., Evolutionary relationships among pathogenic Candida species and relatives (1991) J Bacteriol, 173, pp. 2250-2255Hendricks, L., Goris, A., Neefs, J., Van De Peer, Y., Hennebert, G., De Wachter, R., The nucleotide sequence of the small ribosomal subunit RNA of the yeast Candida albicans and the evolutionary position of the fungi among the eukaryotes (1989) System Appl Microbiol, 12, pp. 223-22

    Similar works