Objetive: To estimate the prevalence of HIV infections, as well as hepatits B and C and syphilis viruses in homeless people. Methods: Cross-sectional study with educational intervention, conducted in the city of São Paulo, between 2002 and 2003. A convenience of homeless people who used night shelters was selected, according to the following criteria: aged 18 or older and not showing psychiatric disturbances. During interviews, sociodemographic and behavioral data were gathered and HIV, hepatitis B and C and syphilis laboratorial tests and post-test counseling were carried out. Results: A total of 330 shelter users participated, with an average age of 40.2 years, 80.9% of them male, having lived on the streets for one year in average. Prevalences of 1.8% for HIV, 8.5% for hepatitis C virus, 30.6% for previous hepatitis B infection, 3.3% for chronic or acute infection by hepatitis B virus, and 5.7% for syphilis. The consistent use of condoms was referred to by 21.3% of interviewees and the use of injecting drugs by 3% of them. Positivity was 10% for HIV and 50% for hepatitis C virus among injectable drug users, versus 1.5% for HIV and 7.3% for hepatitis C among the others, showing an association between the virus and the use of injecting drugs. Previous imprisonment was referred to by 7.9% of women and 26.6% of men, with a prevalence of 2.6% for HIV and 17.1% for hepatitis C virus. Conclusions: The high prevalences of HIV and hepatitis B and C viruses require prevention programs based on vaccination against hepatitis B, early diagnosis of these infections and placement of homeless people into health services.41SUPPL. 24756Allen, D.M., Lehman, J.S., Green, T.A., Lindegren, M.L., Onorato, I.M., Forrester, W., HIV infection among homeless adults and runaway youth, United States, 1989-1992. 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