Different Lethal Effects By Enzyme-generated Triplet Indole-3-aldehyde In Different Escherichia Coli Strains

Abstract

Strains of Escherichia coli which lack 4-thiouridine (S4U) exhibit a higher survival rate than their wild-type parents which contain S4U after treatment with enzyme-generated triplet indole-3-aldehyde. In a similar manner to results obtained with monochromatic 334 nm UV light, the survival is related to single-strand breakage of DNA in E. coli containing the pBR 322 plasmid. The effects of the excited states generated by an enzymatic system suggest that S4U is an important chromophore in the lethal effects observed. The results also suggest that the energy transferred from triplet indole-3-aldehyde to S4U may also be passed from S4U of T-RNA to DNA, possibly through a singlet oxygen intermediate generated by excited S4U, resulting in a decrease in the survival rate of E. coli containing S4U. These results emphasize the importance of excited states in biological systems. © 1990.44371378Peak, Peak, Nerad, The role of 4-thiouridine in lethal effects and in DNA backbone breakage caused by 334 nm ultraviolet light in Escherichia coli (1983) Photochem. Photobiol., 37, pp. 169-172De Mello, De Toledo, Durán, Photo- and biophotoenergized cycloaddition of indole-3-aldehyde to uridine (1981) Acta Sud Am. Quim., 1, pp. 135-141Durán, Brunet, Gallardo, An experiment in photochemistry α-Oxidation of indole-3-acetic acid catalyzed by peroxidase (1984) Biochemical Education, 12, pp. 173-178Durán, Cadenas, The role of singlet oxygen and triplet carbonyls in biological systems (1987) Reviews of Chemical Intermediates, 8, pp. 147-187De Mello, De Toledo, Haun, Cilento, Durán, Excited indole-3-aldehyde from peroxidase catalyzed aerobic oxidation of indole-3-acetic acid. Reaction with and energy transfer to transfer-ribonucleic acid (1980) Biochemistry, 19, pp. 5270-5275De Mello, de Toledo, Aoyama, Sarkar, Cilento, Durán, Peroxidase-generated triplet indole-3-aldehyde adds to uridine bases and excites the 4-thiouridine group in t-RNA(Phe) (1982) Photochem. Photobiol., 36, pp. 21-24Durán, Marcucci, De Mello, Faljoni-Alario, Enzymatically generated electronically excited molecules induce transformation of 4-thiouridine to uridine (1983) Biochem. Biophys. Res. Commun., 117, pp. 923-929Durán, De Mello, Photobiochemistry of 4-thiouridine in E coli t-RNA (1983) Photochemistry and Photobiology, 37, p. S11De Toledo, Zaha, Durán, DNA strand scission in E. coli by electronically excites state molecules generated by enzymatic systems (1982) Biochem. Biophys. Res. Commun., 104, pp. 990-995Menck, Cabral Neto, Faljoni-Alario, Alcantara-Gomes, Damage induced in λ-phase DNA by enzyme-generated triplet acetone (1985) Mutat. Res., 165, pp. 9-14Guillo, De Toledo, Durán, Attemptet detection by fluorescence probes of DNA modifications produced by bioenergized triplet acetone (1983) Photobiochem. Photobiophys., 6, pp. 177-186Nassi, Schiffmann, Fabre, Adam, Fuchs, Induction of the SOS function sfiA in E. coli by systems which generate triplet ketones (1988) Mutat. Res., 198, pp. 53-60Durán, Marcucci, De Mello, Leite, Faljoni-Alario, DNA modification through biophotoenergized processes on t-RNA (1984) Photochem. Photobiol., 39, p. 80SDurán, Marcucci, Gatti, Leite, Lethal effect and DNA breakage caused by biophotoenergized indole-3-aldehyde in E. coli (1986) Int. Symp. on Free Radicals and Excited State in Biological Systems, Buenos Aires, , Abstract 16Birnboim, Doty, A rapid alkaline extraction procedure for screening recombinant plasmid DNA (1979) Nucleic Acids Research, Z, pp. 1513-1520Durán, Faria-Furtado, Faljoni-Alario, Campa, Brunet, Freer, Singlet oxygen generation from the peroxidase-catalyzed aerobic oxidation of an activated —CH substrate (1984) Journal of Photochemistry, 25, pp. 285-295Hardwick, Von Sprecken, Yielding, Yielding, Ethidium binding sites in plasmid DNA determined by photoaffinity labeling (1984) J. Biol. Chem., 159, pp. 11090-11097Hass, Webb, Photodynamic effects of dyes on bacteria. I. V. Lethal effects of acridine orange and 460 or 500 nm monochromatic light in strains of Escherichiacoli that differ in repair capability (1981) Mutat. Res., 81, pp. 277-285Salet, Bazin, Moreno, Favre, 4-Thiouridine as photodynamic agent (1985) Photochemistry and Photobiology, 41, pp. 617-619Thomas, Favre, 4-Thiouridine triggers both growth delay induced by near-ultraviolet light photoprotection (1980) Eur. J. Biochem., 113, pp. 825-834Tsai, Jagger, The roles of the Rel(+) gene and of 4-thiouridine in killing photoprotection of Escherichia coli by near ultraviolet radiation (1981) Photochem. Photobiol., 33, pp. 825-834Caldeira de Araujo, Favre, Near ultraviolet DNA damage induces the SOS response in Escherichia coli (1986) EMBO J., 5, pp. 175-179Peak, Peak, MacCoss, DNA breakage caused by 334 nm ultraviolet light enhanced by naturally occurring nucleic acid components nucleotide coenzymes (1984) Photochem. Photobiol., 39, pp. 713-716Peak, Peak, Foote, Observation on the photosensitized breakage of DNA by 2-thiouracil and 334 nm ultraviolet radiation (1986) Photochem. Photobiol., 44, pp. 111-116Town, Smith, Kaplan, DNA polymerase required for rapid repair of X-rays-induced DNA strand breaks in vivo (1971) Science, 171, pp. 851-854Smith, Sargentini, Metabolically produced UV-like DNA damage and its role in spontaneous mutagenesis (1985) Photochem. Photobiol., 42, pp. 801-803Durán, Campa, Leite, Cilento, Cadenas, Microsomal lipid peroxidation concomitant to peroxidase-catalyzed aerobic oxidation of indole-3-acetate (1986) Photobiochem. Photobiophys., 11, pp. 281-29