Gel Immobilization Of Enzymes From Malted Corn [imobilização De Enzimas De Milho Maltado Em Gel]

Abstract

This work aimed at optimizing the immobilization of amylases obtained from corn malt by the occlusion method with sodium alginate. Corn malt concentration, sodium alginate mass percentage and pH were used as factors that influence enzyme immobilization. The results showed that the best immobilization conditions were obtained at two ranges of corn malt concentration, one between 3.75-5 g.L-1 and the other between 15-16.25 g.L-1, at pH between 4.83-6.6 and at 4% (m/m) sodium alginate. In these conditions, 100% of the enzymes were immobilized with a low loss of activity. His work showed how to obtain immobilized corn malt amylases that can be used in industrial processes of starch hydrolysis by occlusion in sodium alginate.283642648BARROS NETO, B.SCARMINIO, I. S.BRUNS, R. E. Como Fazer Experimentes: Pesquisa e Desenvolvimento na Ciência e na Indústria. 1a ed. Campinas: EDUNICAMP, 2001. (Coleção Livros Textos)BARROS NETO, B., SCARMINIO, I.S., BRUNS, R.E., Planejamento e Otimização de Experimentos, l ed (1995) Série Manuals, , Campinas: EDUNICAMPBAYRAMOGLU, G., YILMAZ, M., ARICA, Y., Immobilization of a thermostable α-amylase onto reactive membranes: Kinetics characterization and application to continues starch hydrolysis (2004) Food Chemistry, 84 (4), pp. 591-599BIAZUS, J.P.M., Otimização da secagem do malte de Zea mays. (2006) Ciência e Tecnologia de Alimentos, 26 (4), pp. 787-792BIAZUS, J.P.M., Caracterização da atividade amilásica do malte de milho (Zea mays). (2006) Acta Scientiarum, 28 (1), pp. 13-19BIAZUS, J.P.M., Study of amylases recovery from maize malt by ion-exchange expanded bed chromatography (2006) Process Biochemistry, 41 (8), pp. 1786-1791BIAZUS, J.P.M., Maximizing of production stages of malt from Zea mays (2005) Brazilian Journal of Food Technology, v, pp. 138-145. , special, pBIAZUS, J.P.M., Optimization of drying process of Zea mays malt to use as alternative source of amylolytics enzymes (2005) Brazilian Archives of Biology and Technology, 48 (SPEC. ISSUE), pp. 185-190BORZANI, W., (2001) Biotecnologia Industrial: Engenharia Bioquímica, 2. , 1 ed. São Paulo: Edgard Blucher LtdaBRADFORD, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein. Utilizing the principle of protein-dye binding (1976) Analytical Biochemistry, 72 (1), pp. 248-254BRENA, M.B., Biolation and reversible immobilization of sweet potato b-amylase on thiolsulfonate-agarose (1993) Journal of Molecular Catalysis, 84 (3), pp. 381-390DAY, G., SINGH, B., BANERJEE, R., Immobilization of a-Amylase Produced by Bacillus circulant GRS 313 (2003) Brazilian Archives of Biology and Technology, 46 (2), pp. 167-177CASTRO, H.F., ANDERSON, W.A., Fine chemicals by biotransformation using lipases (1995) Química Nova, 18 (6), pp. 544-554CRUZ, A. B. Lipase de farelo de arroz: Extração, imobilização e aplicação. Campinas, 2000, 101p. Tese - (Doutorado em Engenharia de Alimentos), Faculdade de Engenharia de Alimentos, Universidade Estadual de Campinas - UNICAMPDALLA-VECCHIA, R., NASCIMENTO, M.G., SOLDI, V., Aplicações sintéticas de lipases imobilizadas em polímeros. (2004) Química Nova, 27 (4), pp. 623-630GASPARI, J. W.GOMES, L. H.TAVARES, F. C. A. Imobilizac̃ão da inulinase de Kluyveromyces markianus para a hidrolise de extratos de Helianthus tuberosus L. Scientia Agrícola, v. 56, n. 4 suplemento, p. 1135-1140, 1999GOODENOUGH, P., LAW, B., Enzymes for food biotechnology (1991) BFE, 8 (12), pp. 22-24KOURKOUTAS, Y., Immobilization technologies and support materials suitable in alcohol beverages production: A review (2004) Food Microbiology, 21 (4), pp. 377-397LIM, H.L., MACDONALD, D.G., HILL, G.A., Hydrolysis of starch particles using immobilized barley cx-amylase (2003) Biochemical Engineering Journal, 13 (1), pp. 53-62MALCATA, F.X., Immobilized lipase reactors for modification of fats and oils-A review (1990) Journal of the American Oil Chemists Society, 67 (12), pp. 890-910OLSON, N.F., RICHARDSON, T., Immobilized enzymes in food processing and analysis (1974) Journal of Food Science, 39 (3), pp. 653-659PEREIRA, E.B., ZANIN, G.M., CASTRO, H.F., Immobilization and catalytic properties of lipase on chitosan for hydrolysis and esterification reactions (2003) Brazilian Journal of Chemical Engineering, 20 (4), pp. 343-355REGULY, J. C. Biotecnologia dos Processes Fermentativos. 1 ed. Pelotas: EDUFPel, 2000. v. 3ROBLE, N.D., OGBONNA, J.C., TANAKA, H., A novel circulation loop bioreactor with cells immobilized in loofa (Lua cylindrical) sponge for the bioconversion of raw cassawa starch to ethanol (2000) Applied Microbiology Biotechnology, 60 (3), pp. 671-678SANTANA, J. C. C. Recuperação das enzimas e β-amilases em sistema bifásico aquoso PEG/CaCl2 para uso como biocatalizador amiláceos. Campinas, 200. 232p. Dissertaç ão - (Mestrado em Engenharia Química), Faculdade de Engenharia Química, Universidade Estadual de Campinas - UNICAMPSOARES, C. M. F. Otimização por planejamento experimental da imobilização de lipase em silica de porosidade controlada na presença de estabilizante. Campinas, 2000. 107p. Dissertaç ão -(Mestrado em Engenharia de Alimentos), Faculdade de Engenharia de Alimentos, Universidade Estadual de Campinas - UNICAM