Evaluation Of Anti-d Reagents In The Detection Of Weak D And Partial D Antigens [avaliação De Reagentes Anti-d Na Detecção Dos Antígenos D Fraco E D Parcial]

Abstract

Monoclonal antibodies (MoAb) anti-D IgG and IgM are being developed in order to replace polyclonal antibodies. However, there are few studies about the selection of these MoAb to routinely detect weak D and partial D antigens. A total of 56 weak D blood samples were analyzed with anti-D IgG and IgM MoAb in order to evaluate their reactivity. Molecular analyses were also performed to characterize the weak D types and the presence of partial D. Antigen densities were determined by flow cytometry. Weak D type 1, 3 and 4 samples with high antigen density were reactive with the MoAb anti-D IgM at room temperature while weak D type 2 and the partial D samples with low antigen density were detected with MoAb anti-D IgG using the indirect antiglobulin test. Our results show that anti-D IgM does not detect partial D samples reactive as weak D and thus can be used for routine testing of patients. 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