The morphology of spermatozoon of Sceliphron fistularium is very similar to that described for bees. In particular, the response to E-PTA stains is similar to that observed in corbiculated Apidae, especially Meliponini bees. Spermatozoa measure 285 μm and are composed of 1) a bilayered acrosome (acrosomal vesicle and perforatorium); 2) a homogeneous and compact nucleus; 3) a 9+9+2 axoneme; 4) a rod-shaped centriolar adjunct; 5) two asymmetrical mitochondrial derivatives with paracrystalline material exclusively in the larger one, and 6) two accessory bodies. Only the accessory microtubules of axoneme and the paracrystalline material are E-PTA positive. Comparison of S. fistularium sperm to data on Hymenoptera corroborates their proximity with bees.373-4313321Alexandre, B.A., Michener, C.D., Phylogenetic studies of the families of short-tongued bees (Hymenoptera: Apoidea) (1995) Uni. Kan. Sci. Bull., 55, pp. 377-424Baccetti, B., Insect sperm cell (1972) Adv. Insect. Physiol., 9, pp. 315-397Baer, B., Schimid-Hempel, P., Hoeg, J.T., Boomsma, J.J., Sperm length, sperm storage and mating system characteristics in bumble-bees (2003) Ins. Soc., 50, pp. 101-108Baó, S.N., Gonçalves-simões, D.A., Lino-Neto, J., Sperm ultrastructure of the bees Exomalopsis (Exomalopsis) auropilosa Spinola 1853 and Paratetrapedia (Lophopedia) sp. Michener and Moures 1957 (Hymenoptera, Apidae, Apinae) (2004) J. Submicrosc. Cytol. Pathol., 36, pp. 23-28Bohari, R.M., Menke, A.S., (1976) Sphecidae Wasp of the World. A Generic Revision, p. 695. , University of California Press, BerkeleyBrothers, D.J., Phylogeny and classification of the aculeate Hymenoptera, with special reference to the Mutillidae (1975) Uni. Kan. Sci. Bull., 50, pp. 483-648Brothers, D.J., Phylogeny and evolution of wasps, ants and bees (Hymenoptera, Crysidoidea, Vespoidea and Apoidea) (1999) Zool. Scripta, 28, pp. 233-249Cruz-höfling, M.A., Cruz-Landim, C., Kitajima, E.W., The fine structure of spermatozoa from the honey bee (1970) An. Acad. Brás. Ciênc., 42, pp. 69-78Duchateau, M.J., Mariën, J., Sexual biology of haploid and diploid males in the bumble bee Bombus terrestris (1995) Ins. Soc., 42, pp. 255-266Fiorillo, B., Coelho, A.A.M., Lino-Neto, J., Báo, S.N., Structure and ultrastructure of the spermatozoa of Halictidade (2005) J. Submicrosc. Cythol. Pathol., , in pressHanson, P.E., Gauld, I.D., (1995) The Hymenoptera of Costa Rica, , Oxford University Press, New YorkHoage, T.R., Kessel, R.G., An electron microscope study of the process of differentiation during spermatogenesis in the drone honey bee (Apis mellifera L.) with special reference to centriole replication and elimination (1968) J Ultrastruct. Res., 24, pp. 6-32Jamieson, B.G.M., Dallai, R., Afzelius, B.A., (1999) Insects: Their Spermatozoa and Phylogeny, p. 555. , Scientific Publishers, Enfield, NH, USALanham, U.N., Evolutionary origin of bees (1981) J. NY Entomol. Soc., 88, pp. 199-209Lensky, Y., Ben-David, E., Schindler, H., Ultrastructure of the spermatozoa of the mature drone honey bee (1979) J. Apic. Res., 18, pp. 264-271Lino-Neto, J., Báo, S.N., Dolder, H., Structure and ultrastructure of the spermatozoa of Bephratelloides pomorum (Fabricius) (Hymenoptera: Eurytomidae) (1999) Int. J. Insect Morphol. Embryol., 28, pp. 253-259Lino-Neto, J., Báo, S.N., Dolder, H., Structure and ultrastructure of the spermatozoa of Trichogramma pretiosum Riley and Trichogramma atopovirilia Oatman and Platner (Hymenoptera: Trichogrammatidae) (2000) Acta Zool. (Stockholm), 81, pp. 205-211Lino-Neto, J., Báo, S.N., Dolder, H., Sperm ultrastructure of the honey bee (Apis mellifera) (L) (Hymenoptera, Apidae) with emphasis on the nucleus-flagellum transition region (2000) Tissue Cell, 32, pp. 322-327Lino-Neto, J., Dolder, H., Ultrastructural characteristics of the spermatozoa of Scelionidae (HymenopteraPlatygastroidea) with phylogenetic considerations (2001) Zool. Scripta, 30, pp. 89-96Lino-Neto, J., Dolder, H., Redescription of sperm structure and ultrastructure of Trichogramma dendrolimi (Hymenoptera: Chalcidoidea: Trichogrammatidae) (2001) Acta Zool. (Stockholm), 82, pp. 159-164Lino-Neto, J., Dolder, H., Sperm structure and ultrastructure of the fire ant Solenopsis invicta Bauren (Hymenoptera, Formicidae) (2002) Tissue Cell, 34, pp. 124-128Lomholdt, O., On the origin of the bees (1982) Entomol. Scand., 13, pp. 185-190Melo, G.A.R., Phylogenetic relationships and classification of the major lineages of Apoidea (Hymenoptera), with emphasis on the crabronid wasps (1999) Uni. Kan. Sci. Bull., 14, pp. 1-55Michener, C.D., Family-group names among bees (1986) J. Kan. Ent. Soc., 59, pp. 219-234Michener, C.D., (2000) The Bees of the World, , Johns Hopkins University Press, Baltimore and LondonNewman, T.M., Quicke, D.L.J., Sperm development in the imaginal testes of Aleiodes coxalis (Hymenoptera: Braconidae: Rogadinae) (1998) J. Hym. Res., 7, pp. 25-37Newman, T.M., Quicke, D.L.J., Ultrastructure of imaginal spermatozoa of sawflies (Hymenoptera: Symphyta) (1999) J. Hym. Res., 8, pp. 35-47Newman, T.M., Quicke, D.L.J., Ultrastructure of spermatozoa in Leptopilina (Hymenoptera: Cynipoidea: Eucoilidae) (1999) J. Hym. Res., 8, pp. 197-203Newman, T.M., Quicke, D.L.J., Sperm development and ultrastructure of mature spermatozoa of Megalyra (Hymenoptera: Megalyroidea) (2000) J. Hym. Res., 9, pp. 62-70Peng, C.Y.S., Yin, C.M., Yin, L.R.S., Effect of rapid freezing and thawing on cellular integrity of honey bee sperm (1992) Physiol. Entomol., 17, pp. 269-276Peng, C.Y.S., Yin, C.M., Yin, L.R.S., Ultrastructure of honey bee, Apis mellifera sperm with special emphasis on the acrosomal complex following high-pressure freezing fixation (1993) Physiol. Entomol., 18, pp. 93-101Quicke, D.L.J., Ingram, S.N., Baillie, H.S., Gaitens, P.V., Sperm structure and ultrastructure in the Hymenoptera (Insecta) (1992) Zool. Scripta, 21, pp. 381-402Rothschild, L., The spermatozoa of the honey bee (1955) Trans. Roy. Ent. Soc. (London), 107, pp. 289-294Silveira, F.A., Melo, G.A.R., Almeida, E.A.B., (2002) Abelhas Brasileiras: Sistemática e Identificação, p. 253. , IDM Composição e Arte, Belo HorizonteWheeler, D.E., Crichton, E.D., Krutzsch, P.H., Comparative ultrastructure of ant spermatozoa (Formicidae: Hymenoptera) (1990) J. Morphol., 206, pp. 343-350Zama, U., (2003) Estudo Estrutural e Ultraestrutural Dos Espermatozóides Nas Tribos Apini, Bombini, Euglossini e Meliponini (Hymenoptera: Apinae), Com Considerações Filogenéticas, , PhD thesis. State University of Campinas, BrazilZama, U., Lino-Neto, J., Dolder, H., Ultrastructure of spermatozoa in Plebeia (Plebeia) droryana Friese (Hymenoptera: Apidae: Meliponina) (2001) J. Hym. Res., 10, pp. 261-270Zama, U., Lino-Neto, J., Dolder, H., Structure and ultrastructure of spermatozoa in Meliponini (Stingless bees) (Hymenoptera: Apidae) (2004) Tissue Cell, 36, pp. 29-41Zama, U., Lino-Neto, J., Melo, S.M., Campos, L.A.O., Dolder, H., Ultrastructural characterization of spermatozoa in Euglossini bees (Hymenoptera: Apidae: Apinae) (2005) Ins. Soc., 52, pp. 122-13
