PENGARUH MARMIN, SENYAWA AKTIF Aegle marmelos Correa TERHADAP OTOT POLOS TRAKEA MARMUT TERISOLASI

Abstract

Marmin has a good chance as an anti-allergic agent. Marmin can be used to diminish allergy symptoms, including contraction of the airways. Pharmacodynamics effects of marmin on various receptors of airways have not been studied yet. The purposes of this study were to evaluate the activities of marmin as H1 and Ach-M3- receptor antagonists, and β2-adrenergic receptor agonists. In addition, the study also investigated marmin molecular mechanisms in inhibiting smooth muscle contraction through preventing elevated-levels of isolated guinea-pig tracheal smooth muscle intracellular Ca2+. The study was performed using a guinea-pig tracheal smooth muscle in Krebs buffer solution (isolated organ method). Histamine and metacholine were used as agonists for H1 and Ach-M3 receptors respectively. Isoproterenol, a β2-adrenergic receptor agonist was also used to induce tracheal smooth muscle relaxation in Krebs buffer solution. Membrane depolarization and stimulating activation of voltagedependent Ca2+-channels were induced by KCl. The result showed that marmin (10 and 100 μM) has a reversible competitive antagonist to H1 receptor with pA2 value of 2.302. In contrast, marmin provides a reversible non-competitive antagonist on Ach-M3 receptors. Marmin did not affect isoproterenol-induced β2-adrenergic receptor. Furthermore, marmin inhibited extracellular Ca2+ influx and Ca2+ release from a calcium-store. Additionally, marmin inhibited intracellular Ca2+ elevation and decreased the ability of metacholine-induced tracheal smooth muscle contraction