Studi pathologis cytopathological effect bovine herpes virus 1 penyebab infectious bovine rhinotracheitis dari sapi pada telur ayam berembrio

Abstract

The objective of this study was to investigate about cytopathological effect (CPE) of Bovine Herpes Virus 1 that caused the Bovine Rhinotracheitis Infections (IBR) from bovine in the corioallantoic membrane (CAM) of the embryoned chicken egg (ECE). Seven of swab nasal samples were taken from the bovine in the Breeding Farm of Padang Mengatas, West Sumatera. They were inoculated 0,5 ml in the corioallantoic membrane of the 10 old days embryoned chicken egg with thirth repetitively. Before that, the samples were given antibiotic and antifungi treatment to protect from secunder infection. The observing was conducted first day until thirth day after infection. It was done to observe the pathological changes in the corioallantoic membrane of embryoned chicken egg. The result showed that there was a cytopathological effect as pock lesion in CAM with variety size. The histopathologic changes appeared an asidofilic nuclear inclution body in the epithel of membrane. It showed that the virus could replicate in the nuclear of CAM. So, The conclusion of this study was a IBR virus has a cytopathological effect in CAM of ECE with a specific pock lesion. Key words : CAM, IBR, ECE, pock lesio