UJI SITOTOKSISITAS POLYETHYLENE FIBER REINFORCED COMPOSITE PADA SEL FIBROBLAS (METODE MTT)

Abstract

Fiber reinforced composite (FRC) is material consist matrix which is reinforced by fiber. Polyethylene fiber is a fiber that is commonly used in dentistry, because of its good mechanical properties. Materials used in dentistry must have good biocompatibility. The aim of this study was to determine cytotoxicity of polyethylene FRC on fibroblasts cells. The materials used were polyethylene fiber (CONSTRUCT KerTM, USA) and resin composite Masterflow (Biodinamica, Brazil). There were two research groups. The first group was composite resin and the second was polyethylene FRC. Each material inserted into the mold (2x2x25mm) and cured by visible light cure for 20 seconds, then powdered. Vero cell line (LPPT, UGM), were cultured and inserted into the wells with density 2x104 sel/100μL, then incubated for 24 hours at 37oC. Composite and polyethylene FRC powder were dissolved using culture medium, and then inserted into the cells and incubated. MTT solution added into the the cells, then incubated for 4 hours. SDS-HCL added into each well. Optical Density value obtained from the amount of formazan crystals formed using ELISA plate reader with wavelength of 550 nm. The percentage of cell viability of each sample was calculated by comparing the optical density treatment and control. The data was statistically tested by t-test. Average of percentage cell viability were exposed by resin composite was 83.108+4.113% and polyethylene FRC was 87.463+4751%. The result of t-test showed that there was no significant difference between the two groups (P> 0.05%). The conclusion of this study was polyethylene fiber reinforced composite was not cytotoxic to fibroblast. Keywords: FRC, polyethylene, cytotoxicity, biocompatibility, MTT assa