The performance of Cardio Pulmonary Resuscitation (CPR) chest compressions on a patient provide critical blood flow and oxygen delivery to the heart and brain. The objective of this study was to establish the relationship between the rate of muscle fatigue and chest compression performance. It was hypothesized that if performance of chest compressions cause fatigue after five minutes, then the rate of muscle fatigue will be indicated through surface electromyography (sEMG) measurements. Four participants (M=2, F=2) volunteered to perform chest compressions on a CPR mannequin. sEMG electrodes were placed on the participants’ dominant limb anterior deltoid muscle to capture muscle activity while performing chest compressions. Each participant completed four sets of CPR chest compressions with a two minute rest period between sets. Data was analyzed through the iWrox LabScribe™ software. The absolute interval, root mean square, and minimum and maximum amplitudes for each signal were collected for data analysis. The mean amplitude percent decline was calculated for each set of chest compressions in three-minute intervals for each participant. Examination of the rate of decline between subjects, indicating muscle fatigue was evident. The hypothesis was therefore accepted; there was convincing muscle fatigue shown through sEMG measurements while CPR chest compressions were performed. If the study was to be replicated, the author recommends that the study involve more participants to confirm the established data