[概要]希土類元素--タンパク質--ドデシルベンゼンスルホン酸ナトリウム(SDBS)系における蛍光増感効果を調べた。Tb^3+-SDBS-タンパク質系の蛍光は大変弱い。しかしながら、この系にGd^3+やエタノールを加えると、蛍光が著しく増加した。 コラーゲンの場合には、この効果が最大であった。この結果をもとに、タンパク質をナノグラム/mlレベルで感度良く検出する方法を確立した。 [Abstract] The fluorescence enhancement effect in rare earth-protein-sodium dodecyl benzenesulfonate (SDBS) system has been studied. The photoluminescence of Tb^3+-SDBS-protein system was very weak. However, when Gd^3+or ethanol was added into this system, the fluorescence was significantly enhanced. In the case of collagen, this effect was greatest. Based on this, a method was established to determine proteins sensitively at nanogram/ml level
