This study aims to analyze the characteristics and perceptions of service users on the use of health centers as gatekeepers in Sragen Regency. The sample in this study amounted to 105 respondents from public health centers in Sidoharjo, Sragen Regency using a questionnaire for data collection. Sampling technique with non-probability sampling method. The data analysis technique uses path analysis. The results of this study indicate that: 1) There is a relationship between differences in age, education, occupation, chronic disease, attitude of officers, length of treatment with the perception of service users using the use of health centers as gatekeepers in Sragen Regency 2) There is an influence of perceptions of health service quality on the use of health centers as gatekeepers in Sragen Regency