Background: The high stress level is caused working frquency. Overburden working nurses will cause stress on nurses. This study aims to find relationship between workload with nurse stress level in isolation room at Rumah Sakit Umum Daerah Kota Salatiga. Methods: This study used qualitative method with cross sectional approach. The sample were taken with sampling total technique. The sample amount were 41 respondents. The data analysis of the study used spearman rank test. Research Results: The study found out that the workload in the isolation room are in average experiencing low workload of 36,6 % from 41 nurses, the stress level on isoation room nurses in average experiencing high stress with 34,1%, the relationship of workload with stress showed a significant corelation. It is found that high workloads correlated to high stress level. Therefore, there is correlation betweeen workload with stress level of Covid-19 isolation room nurses in RSUD of Salatiga. This study showed Rho as 0,638 with 0,001 probablity. The probability value showed that there is hypothesis of the significant workload of nurses with their stress level in isolation room of RSUD Salatiga. Conclusion: The findings shows the significant relationship of nurses workload with work stress level in isolation room of RSUD Salatiga
