At present, minimally invasive interventions become more widespread for treating hepatopancreatoduodenal area pathologies. However, new methods and approaches are necessary for obtaining more diagnostic information in real time. Several methods within the framework of "optical biopsy" concept are considered. The features and areas of application of each method are reviewed to find out which of them can be used in further studies to assess the possibility of intraoperative use in minimally invasive abdominal surgery. Preliminary measurements with fluorescence spectroscopy method have been performed at excitation wavelengths 365 nm and 450 nm. Areas of interest were common bile duct, gallbladder and liver abscess. In our opinion, the obtained results can be a basis for further research and provide a deeper understanding of pathological processes of abdominal cavity organs tissues
