Adachi, Lauren Naomi Spezia

Caumo, Wolnei

Fregni, Felipe

Macedo, Isabel Cristina de

Nunes, Éllen Almeida

Oliveira, Carla de

Scarabelot, Vanessa Leal

Souza, Andressa de

Torres, Iraci Lucena da Silva

Vercelino, Rafael

Portuguese

Lume 5.8

35ª SEMANA CIENTÍFICA DO HOSPITAL DE CLÍNICAS DE PORTO  78 Clin Biomed Res 2015; 35 (Supl.)    P 3477 Hyperalgesia is prevented by transcranial direct current stimulation (tDCS) in rats subjected to model chronic stress Carla de Oliveira1,2,4, Isabel Cristina de Macedo1,3,4, Lauren Naomi Spezia Adachi1,2,4, Vanessa Leal Scarabelot1,3,4, Andressa de Souza1,4, Rafael Vercelino1,3,4, Éllen Almeida Nunes1,3,4, Felipe Fregni6, Wolnei Caumo2,5, Iraci Lucena da Silva Torres1,2,3,4,5 1Laboratório de Farmacologia da Dor e Neuromodulação: Investigações Pré-Clínicas- Departamento de Farmacologia – UFRGS.   2 Programa de Pós-Graduaçăo em Medicina: Ciências Médicas – UFRGS. 3 Programa de Pós –Graduação em Ciências Biológicas: Fisiologia – ICBS/UFRGS. 4 Unidade de Experimentação Animal - GPPG - Hospital de Clínicas de Porto Alegre – UFRGS. 5 Departamento de Farmacologia – ICBS/UFRGS. 6Associate Professor of Physical Medicine and Rehabilitation, Associate Professor of Neurology Harvard Medical School. Berenson-Allen Center for Noninvasive Brain Stimulation, Department of Neurology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, United States of America. Hospital de Clínicas de Porto Alegre (HCPA)   Objective: Chronic stress has been related with decreased pain thresholds and hyperalgesia. Additionally, transcranial direct current stimulation (tDCS) induces cortical excitability and has been suggested as a treatment for pain. This study aimed test whether tDCS can  reverse the  specific  behavioral effects of  chronic stress in the pain system. We evaluated also BDNF and IL10 levels. Methods: 24 male Wistar rats were divided in 4 groups: control-TC; chronic stress-S; chronic stress + sham tDCS-SSham and chronic stress + tDCS- StDCS. The animals were exposed to 20-min sessions of 500 μA anodal tDCS, and 30-sec of sham procedure for 8 days before exposure to chronic stress. Chronic stress consisted of 1h per day/5 days on week /11 weeks. After 11 weeks the Hot Plate-HP and Tail-Flick-TF test were done to assess hyperalgesia, and were evaluated the serum levels of BDNF and IL-10. Statistical analysis was performed by One-Way (ANOVA/SNK, p<0.05). Project was approved by Ethics Committee of CEUA/HCPA: 11-0544. Results: The HP final test showed differences (p<0.001), and the tDCS group increased the latency in relation S and Sham groups suggesting reversal of hyperalgesia. TF latency showed decreased in groups that received chronic stress when compared to control (p<0.001). Both, BDNF and IL-10 serum levels not showed significant differences between groups (p>0.05). Conclusion: The chronic stress led to decreased of nociception threshold shown by HP test, suggesting hyperalgesia and this parameter was prevented by tDCS on TF test. Nonetheless, the chronic stress not affected the BDNF or IL-10 serum levels. Keywords: tDCS; hyperalgesia; chronic stress. Projeto 11-0455. Financial Support: FIPE/GPPG-HCPA, CNPq, CAPES.      

Hyperalgesia is prevented by transcranial direct current stimulation (tDCS) in rats subjected to model chronic stress

https://lume.ufrgs.br/bitstream/10183/140807/1/000982995.pdf

Hyperalgesia is prevented by transcranial direct current stimulation (tDCS) in rats subjected to model chronic stress

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