Caumo, Wolnei

Dalmaz, Carla

Fontella, Fernanda Urruth

Nonose, Yasmine

Rozisky, Joanna Ripoll

Santos, Vinícius Souza dos

Torres, Iraci Lucena da Silva

Vendite, Deusa Aparecida

English

Lume 5.8

32ª SEMANA CIENTÍFICA DO HOSPITAL DE CLÍNICAS DE PORTO ALEGRE Rev HCPA 2012; 32 (Supl.)                                                                                         164          NEONATAL MORPHINE TREATMENT ALTERS GLUTAMATE UPTAKE IN SPINAL CORD OF RATS Vinicius Souza dos Santos, Joanna Ripoll Rozisky, Yasmine Nonose, Fernanda Urruth Fontella, Deusa Aparecida Vendite, Carla Dalmaz, Wolnei Caumo, Iraci Lucena da Silva Torres  Introduction: infants that receive opioids in ICU develop symptoms of opiate withdrawal, such as antinociceptive tolerance. We previously showed that morphine exposure in early life promotes a hyperalgesic response to noxious events in adult  life of rats that was reverted by NMDA antagonist receptor. Thus, it suggests that early morphine exposure can be lead to changes in the glutamatergic system. The aim of this study was to determine if morphine administration in early life, once a day for 7 days, alters glutamate uptake in spinal cord synaptosomes. Materials and Methods: each animal received saline (C) or morphine (5 µg s.c. in the mid-scapular area; M) starting at postnatal day 8 (P8) until P14, once a day. At P30 and P60 animals were killed by decapitation and spinal cords were removed to determination of Na+-dependent high-affinity glutamate uptake, as described by Leal et al (2001) (n=3-4/group). The results were expressed as mean + SEM of pmol/min/mg protein. Statistical analysis was performed by Student’s t test, and was considered significant if  P<0.05. This work was approved by Ethical Committee  of HCPA (GPPG 08345). Results: morphine group showed a decreased glutamate uptake in synaptosomes of spinal cord compared to control group at P30 (C=0.3509+0.1244; M=0.0477+0.01347;  F(1,6)=4.746; Student’s  t  test,  P<0.05) and at P60 (C=0.04289+0.009; M=0.01345+0.003;  F(1,5)=5.771; Student’s  t  test,  P<0.05). Conclusion: this  study showed that  morphine  exposure in  early life alters glutamateuptake in spinal cord at medium and long-term after end of treatment. In this way, we can suggest that this  gluta-matergic changes can contributes to hyperalgesia induced by morphine treatment in neonatal period.  

Neonathal morphine treatment alters glutamate uptake in spinal cord of rats

https://lume.ufrgs.br/bitstream/10183/70749/1/000877904.pdf

Neonathal morphine treatment alters glutamate uptake in spinal cord of rats

Abstract

Similar works

Full text

Available Versions

Lume 5.8