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research
Automating the application of smart materials for protein crystallization
Authors
Asanithi
Asherie
+29 more
Caffrey
Chayen
Curcio
D'Arcy
D'Arcy
EL-Sharif
Frenkel
Georgiev
Gorrec
Hazim F. EL-Sharif
Kertis
Khurshid
Lata Govada
Liao
McPherson
Naomi E. Chayen
Newman
Nollert
Page
Rong
Sahir Khurshid
Saridakis
Saridakis
Shaw Stewart
Shim
Subrayal M. Reddy
Sugahara
Thakur
Zheng
Publication date
1 January 2015
Publisher
'International Union of Crystallography (IUCr)'
Doi
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on
PubMed
Abstract
The fabrication and validation of the first semi-liquid nonprotein nucleating agent to be administered automatically to crystallization trials is reported. This research builds upon prior demonstration of the suitability of molecularly imprinted polymers (MIPs; known as 'smart materials') for inducing protein crystal growth. Modified MIPs of altered texture suitable for high-throughput trials are demonstrated to improve crystal quality and to increase the probability of success when screening for suitable crystallization conditions. The application of these materials is simple, time-efficient and will provide a potent tool for structural biologists embarking on crystallization trials. © 2015, IUCR. All rights reserved
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