Iranian Association of Environmental Health (IAEH)
Abstract
Amongst organophosphate pesticides, the one most widely used and common
environmental contaminant is diazinon; thus methods for its trace
analysis in environmental samples must be developed. Use of diazinon
imprinted polymers such as sorbents in solid phase extraction, is a
prominent and novel application area of molecular imprinted polymers.
For diazinon extraction, high performance liquid chromatography
analysis was demonstrated in this study. During optimization of the
molecular imprinted solid phase extraction procedure for efficient
solid phase extraction of diazinon, Plackett-Burman design was
conducted. Eight experimental factors with critical influence on
molecular imprinted solid phase extraction performance were selected,
and 12 different experimental runs based on Plackett-Burman design were
carried out. The applicability of diazinon imprinted polymers as the
sorbent in solid phase extraction, presented obtained good recoveries
of diazinon from LC-grade water. An increase in pH caused an increase
in the recovery on molecular imprinted solid phase extraction. From
these results, the optimal molecular imprinted solid phase extraction
procedure was as follows: solid phase extraction packing with 100 mg
diazinon imprinted polymers; conditioning with 5 mL of methanol and 6
mL of LC-grade water; sample loading containing diazinon (pH=10);
washing with 1 mL of LC-grade water, 1 mL LC- grade water containing
30% acetonitrile and 0.5 mL of acetonitrile, respectively; eluting with
1 mL of methanol containing 2% acetic acid. The percentage recoveries
obtained by the optimized molecular imprinted solid phase extraction
were more than 90% with drinking water spiked at different trace levels
of diazinon. Generally speaking, the molecular imprinted solid phase
extraction procedure and subsequent high performance liquid
chromatography analysis can be a relatively fast and proper approach
for qualitative and quantitative analysis of diazinon in drinking
water