Reconstitution and localisation
studies of a type IV secretion
system
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Abstract
Bacterial conjugation is the transport of a DNA molecule from a donor cell to a
recipient. Since bacteria do not reproduce sexually, conjugation is a major
contributor to prokaryotic genome plasticity and the spread of antibiotic
resistance genes.
A Type IV Secretion System (T4SS) mediates the DNA transport during
conjugation. T4SSs are large macromolecular assemblies embedded in the
membrane of bacteria, and are associated with pathogenesis, bacterial
conjugation and natural transformation. They are a versatile family of secretion
systems, who transport a wide variety of substrates, such as virulence proteins,
DNA––protein complexes as well as only DNA.
Here, we investigate the minimal requirements for conjugation, and the T4SS’s
localisation within the cell. We show that the conjugative T4SS of the plasmid
pR388 requires a total of 14 genes to efficiently mobilise DNA from a donor
cell to a recipient cell and is arranged around the cell circumfence in a helical
array.
Our study of two reconstituted and fully functional conjugative T4SSs opens
doors for further structural and functional analysis