Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Doi
Abstract
Purpose: To investigate the effect of ethyl acetate extract of S.
striata on pro-inflammatory production by macrophages. Methods: Mouse
peritoneal macrophages were cultured in solvent either alone or with 2
µg/ml lipopolysaccaride (LPS) with/without different doses of
ethyl acetate extract of S. striata. Production of pro-inflammatory
cytokines including interleukin 1β (IL-1β), tumor necrosis
factor - a (TNF-a) and prostaglandin E2 (PGE2) were examined using
ELISA. Results: Ethyl acetate fraction of S. striata in doses of 10,
50, 100 and 200 µg/ml significantly (p<0.05) inhibited
pro-inflammatory mediators (IL-1β, TNF-a, and PGE2) production by
LPS stimulated peritoneal macrophages. Conclusion: The
anti-inflammatory effect of the extract on pro-inflammatory cytokines
may ameliorate inflammatory diseases, possibly via an immunomodulatory
mechanism