Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Abstract
Purpose: To develop simple, rapid and selective spectrophotometric
methods for the determination of cilostazol in tablet dosage form.
Methods: Cilostazol was dissolved in 50 % methanol and its absorbance
was scanned by ultraviolet (UV) spectrophotometry. Both linear
regression equation and standard absorptivity were calculated and both
methods were validated as per ICH guidelines. Cilostazol was determined
in tablet dosage form using these validated methods. Results: The
λmax of cilostazol was 258.2 nm in 50 % methanol.
Beer-Lambert’s law was obeyed in the concentration range of 0
– 25 μg/ml and standard absorptivity was 420.2 dL.g-1.cm-1 .
The numerical values for all the validation parameters were within
acceptable limits. The results of cilostazol tablet determination by
linear regression equation and standard absorptivity methods indicate
purity of 100.0 - 102.4 and 98.7 - 101.1 % with standard deviations of
0.611 and 0.592, respectively. Comparing the methods at 99 % confidence
limit, the F-test value was found to be 1.065. Conclusion: These
validated methods may be useful for routine analysis of cilostazol as
bulk drugs, in dosage forms as well as in dissolution studies in the
pharmaceutical industry