We utilized two-dimensional gel electrophoresis and immunoblotting
(2D-immunoblotting) with anti-Sporothrix schenckii antibodies to
identify antigenic proteins in cell wall preparations obtained from the
mycelial and yeast-like morphologies of the fungus. Results showed that
a 70-kDa glycoprotein (Gp70) was the major antigen detected in the cell
wall of both morphologies and that a 60-kDa glycoprotein was present
only in yeast-like cells. In addition to the Gp70, the wall from
filament cells showed four proteins with molecular weights of 48, 55,
66 and 67 kDa, some of which exhibited several isoforms. To our
knowledge, this is the first 2D-immunoblotting analysis of the S.
schenckii cell wall