We show for the first time that the ventral diverticulum of the
mosquito gut (impermeable sugar storage organ) harbors microorganisms.
The gut diverticulum from newly emerged and non-fed Aedes aegypti was
dissected under aseptic conditions, homogenized and plated on BHI
medium. Microbial isolates were identified by sequencing of 16S rDNA
for bacteria and 28S rDNA for yeast. A direct DNA extraction from Ae.
aegypti gut diverticulum was also performed. The bacterial isolates
were: Bacillus sp., Bacillus subtilis and Serratia sp. The latter was
the predominant bacteria found in our isolations. The yeast species
identified was Pichia caribbica