In molecular biology, transformation using E. coli as a host plays a
key role in synthesizing gene libraries. The present study demonstrated
a new ethanol-based method for transformation of plasmid DNA to E.
coli. Ethanol at 10% concentration (v/v) showed best results. Further,
as compared with traditional CaCl2 method, the transformation rate,
using protocol outlined in this study, was very high, suggesting
amenable for further applications