Medknow Publications on behalf of the Association of Radiation Oncologists of India (AROI)
Abstract
Context: Fascin is an actin-bundling protein that has been shown to be
overexpressed in various carcinomas, but the effect of doxorubicin on
its protein and mRNA has not been investigated. Aims: The aim of the
present study is to evaluate any changes that may be induced by
doxorubicin on the fascin molecule in a cancer cell line. Settings and
Design: Experimental study. Materials and Methods: The expression of
fascin protein and mRNA in the human KB carcinoma cell line was
evaluated by qRT-PCR, and immunocytochemistry following treatment with
doxorubicin. Apoptosis was also analyzed using flow cytometry.
Statistical Analysis Used: Group comparisons were performed using
one-way analysis of variance (ANOVA) followed by Tukey's post hoc test
to determine differences between groups (P < 0.05). Results:
Treatment with doxorubicin promoted a significant increase of fascin
mRNA and decrease of fascin protein (P <0.05). Doxorubicin also
induced apoptosis in the KB cell line. Conclusions: Regarding the
changes in the actin-bundling protein, fascin, which was found
following treatment with doxorubicin, it can be hypothesized that this
drug may possess antimigratory characteristics by influencing fascin.
Further studies are suggested to clarify this theory
