Medknow Publications on behalf of Indian Society of Human Genetics
Abstract
HLA antigens were used as markers to establish the presence of
chimerism (i.e. simultaneous presence of two lymphocyte populations
from recipient as well as donor) in a patient with chronic
granulomatous disease treated with one haplotype matched stem cell
transplant. Neutrophil engraftment occurred on Day 6 post peripheral
blood stem cell transplant (PBSCT). Platelet counts were maintained
above 20x10[9]/L. Six months after the allogenic PBSCT, lymphocyte
population was chimeric and cells of both donor (father) and host HLA
type were present. The patient revealed a shift in his HLA antigen
profile and there was evidence of donor cell engraftment. The HLA
phenotype A26,CwXX,B8,DRB1*03//A32,Cw4,B35,DRB1*16// represented his
true phenotype whereas A11,Cw7,B62,DRB1*14 represented donor (father)
origin.. HLA system as a genetic marker is a useful additional approach
to determine engraftment following an allogenic haplo-identical stem
cell transplantation
