Medknow Publications on behalf of Indian Society of Human Genetics
Abstract
Gastric biopsy samples obtained from 14 patients with upper abdominal
pain, clinically diagnosed as acid peptic disease, were analysed for
the presence of Helicobacter pylori (H. pylori) by Polymerase Chain
Reaction (PCR) using partially (template A) and completely purified DNA
(template B). Antigen specific primer was used to analyse the sample by
PCR method. The presence of H. pylori in the samples was confirmed by
running a positive control. The presence of H. pylori was also
detected by urease method using standard protocol. Among the 14 samples
studied, 8 showed the presence of H. pylori with both templates A and
B. Among these 8 samples only 3 showed positive for the presence of H.
pylori with urease method. The present work discusses the results
obtained in the detection of H. pylori in template A and B by PCR
method