Sestre Milosrdnice University hospital and Institute of Clinical Medical Research
Abstract
Dental adhesives come into direct contact with oral tissues. Due to this close and long-term contact, the materials should exhibit a high degree of biocompatibility. The aim of this study was to evaluate the genotoxic effect of dental adhesives on human lymphocytes in vitro. Polymerized dental adhesives (Excite, Adper Single Bond 2, Prompt L-pop and OptiBond Solo Plus) were eluted in dimethyl sulfoxide for 1 hour, 24 h and 120 h (5 days). Thereafter, lymphocyte cultures were treated with different concentrations of eluates (0.2 μg/mL, 0.5 μg/mL and 5 μg/mL) obtained from each of the tested materials. Genotoxicity was evaluated by micronucleus test. The χ2-test was used on statistical analysis (p<0.05). After elution period of 1 h, only the highest dose of all tested materials affected the measured cytogenetic parameters. After 24 h, genotoxicity was demonstrated only in cultures treated with eluates in concentrations of 0.5 μg/mL and 5 μg/mL. Based on the results, it is concluded that the use of dental adhesives causes genotoxic effects in human lymphocytes. Toxic effect of these dental adhesives increases with the tested material concentration and decreases with the length of elution period.Stomatološki dentinski adhezivi dolaze u izravan dodir s oralnim tkivima. Zbog ovog bliskog i dugotrajnog kontakta ove materijale treba obilježavati visok stupanj biokompatibilnosti. Svrha ovoga rada bila je procjena genotoksičnog učinka dentinskih adheziva na ljudskim limfocima in vitro. Polimerizirani adhezivi (Excite, Adper Sngle Bond 2, Prompt L-pop i OptiBond Solo Plus) eluirani su u dimetil sulfoksidu kroz 1 h, 24 h i 120 h (5 dana). Nakon toga su kulture limfocita tretirane različitim koncentracijama eluata (0,2 μg/mL, 0,5 μg/mL i 5 μg/mL) dobivenim iz ispitivanih materijala. Za procjenu genotoksičnosti koristio se mikronukleus test, dok se χ2-test koristio za statističku analizu (p<0,05). Adhezivi pokazuju genotoksičan učinak u kulturi limfocita nakon jednosatnog tretiranja eluatom koncentracije 5 μg/mL. Nakon 24 h svi su ispitivani adhezivi pokazali genotoksičnost u koncentraciji 0,5 μg/mL i 5 μg/mL. Na temelju rezultata može se zaključiti kako upotreba adheziva izaziva genotoksični učinak u ljudskim limfocitima. Toksični učinak povećava se s koncentracijom ispitivanog materijala, a smanjuje s vremenom trajanja eluacije
