Chromogenic detection of yam mosaic virus by closed?tube reversetranscription loop?mediated isothermal amplification (CT?RT?LAMP)

Abstract

AbstractA closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) assay was developed for thedetection of yam mosaic virus (YMV, genus Potyvirus) infecting yam (Dioscorea spp.). The assay uses a set of six oligonucleotideprimers targeting the YMV coat protein region, and the amplification products in YMV-positive samples arevisualized by chromogenic detection with SYBR Green I dye. The CT-RT-LAMP assay detected YMV in leaf and tubertissues of infected plants. The assay is 100 times more sensitive in detecting YMV than standard RT-PCR, while maintainingthe same specificity