Oxidation of 2,5-diketogluconate by a cell-free enzyme preparation from Acetobacter melanogenum

Abstract

IT has been reported by Katznelson that 'old' intact cells and cell-free extracts of Acetobacter melanogenum oxidize glucose to gluconate, 2-ketogluconate and finally to 2,5-diketogluconate. We have obtained evidence that this last compound can be oxidized further