Inchakalody, V

Iyappan, G

Ma, JK-C

Ramalingam, S

Shanmugaraj, BM

St George's Online Research Archive

Abstracts / International Journal of Infectious Diseases 73S (2018) 3–398 363UMP. 742Potential of plant biologics to tackle theepidemic like situations - case studies involvingviral and bacterial candidatesG. Iyappan1,∗, B.M. Shanmugaraj2, V.Inchakalody3, J.K.-C. Ma4, S. Ramalingam51 DRDO-BU Center for Life Sciences, Biotechnology,Coimbatore, India2 Bharathiar University, Biotechnology, India3 Bharathiar University, Biotechnology, India4 St. George’s Hospital Medical School, Institute forInfection and Immunity, London, United Kingdom5 Bharathiar University, Biotechnology, Coimbatore,IndiaBackground:Theart of recombinantproteinproduction inE.coliandmammalian system needs an extensive infrastructure to adoptGMP practice for large-scale production and this makes the ﬁnalproduct very expensive and such therapeutics may not be afford-able where it is needed the most. The potential of plant biologicsover the conventional systems is, it has glycosylation pattern orwith a choice of non-glycosylation containment i.e. in chloroplast,established genetic and glycoengineering methods, adaptability,scalability and affordability. Plant molecular farming in simplewords ‘the use of plants for the production of protein therapeutics’became popular due to the Magnifection technology, degenerativevectors and glycoengineered plants. Our studies involved the usageof current technology for expression and puriﬁcation of Chikun-gunya vaccine candidates and mAb’s in plant system and alsomanifests the compatibility of plant host system for the expressionof S.aureus superantigenic proteins with the proven immunogenicfunctions.Methods & Materials: The CHIKV vaccine candidates E1 and E2,bacterial superantigenic peptides TSST-1 and fusion gene rTE andanti-CHIKV mAb’s (8B10 and 5F10 were retrieved from Europeanpatent application EP2374 816 A1) were cloned into the currentgeneration high expression plant constructs like pEAQ-HT-DESTand pTRAK through gateway cloning technology and conventionalcloning. Constructsweremobilized intoAgrobacterium andvacuuminﬁltrated in N.benthamiana plants following standard procedures.Following 5th day of post inﬁltration leaves were harvested andrecombinantproteinwaspuriﬁedbyhis-tagandproteinA/Gafﬁnitybased puriﬁcation techniques respectively.Results: We have successfully produced CHIKV vaccine can-didates E1 and E2 (8-13mg/kg of fresh leaf weight), antibodies(20-30mg/kg) and the bacterial recombinant superantigen TSST-1 and hypothetical vaccine candidate rTE fusion proteins (5.4 and6.7mg/kg). The in vivo and in vitro immunogenicity and neutral-ization studies revealed that the plant produced antigens andantibodies elicit good immune responses.Conclusion: The level of expression, glycosylation and immu-nizing potential are the evidence strongly suggesting the plants arethe potential alternate system. So, we propose plant biologics as apromising system during an epidemic like situation as scale-up ofthis system is inﬁnity at an affordable cost, which will help thepeople, where it is needed the most.https://doi.org/10.1016/j.ijid.2018.04.4236

Potential of plant biologics to tackle the epidemic like situations - case studies involving viral and bacterial candidates

http://openaccess.sgul.ac.uk/110128/1/Potential_of_plant_biologics.pdf

Potential of plant biologics to tackle the epidemic like situations - case studies involving viral and bacterial candidates

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