Enhanced bone marrow derived mesenchymal stem cell differentiation when isolated and expanded with human platelet rich plasma and differentiation media is supplemented with vitamin D
Vitamin D3 is well known to be involved in bone formation during foetal development and has been shown to be actively involved from gestational day 13 during foetal rat development [1]. Vitamin D3 and its associated enzyme 1-alpha-hydroxylase a member of the cytochrome P450 super family and encoded for by the gene CYP27B1. In this study we supplemented osteogenic media with vitamin D at a concentration of 10-4M and differentiated for 21 days with samples analysed by means of quantitative alizarin red assay and qPCR for the bone markers RUNX2, ALPL and HPRT
