Through years of technology development, the drug industry has been able to synthesize many valuable medicines to provide better health care. However, any time a new medication (or industrial chemical for that matter) is made; it must be tested to ensure that it is not carcinogenic. For years, scientists have worked to design a screening method that is fast, efficient, and reliable. Currently, the most widely-used method used is the Ames test [1]. This test has both strengths and weaknesses which will be discussed. In this project, Two Dimensional Electrophoresis (2DE) is used to monitor protein expression in Pseudomonas putida F1 grown on different carbon sources with the purpose of finding a set of carcinogenic indicator proteins, which will lead to a replacement for Ames test. 2DE provides a molecular approach to carcinogenesis, thus is more detailed and potentially more reliable. The result of the project as well as possible future directions for this research will be discussed
