A Burgener

A Karlas

Adam Burgener

AL Brass

AL Remoli

Alash'le G. Abimiku

Alexander M. Cole

Amy L. Cole

AR Makela

B Persson

C Benarafa

C Devito

C Morrison

CA Chappell

E Gwyer Findlay

EV Valore

F Ang

F Hattori

Frank Plummer

G Ray

Garrett R. Westmacott

GW Newman

H Hocini

H Nakamura

H Schenk

H Zhou

J Koch

J Munch

J Zhou

JE Embree

JL Shaw

JM Jacque

Joshua Kimani

K Broliden

Kenzie D. M. Birse

KR Fowke

Kristina Broliden

Lyle McKinnon

M Ghosh

M Glaser

M Mazzocco

M Sakaguchi

MA Jendrysik

MV Patel

N Venkataraman

O Martinez

P Laneve

P Levinson

Q Lizeng

R Geiben-Lynn

R Konig

RM Novak

RY Choi

S Morizane

S Rahman

SM Iqbal

SM Wahl

SY Doerflinger

T Hirbod

T Jinquan

T Kouno

Taha Hirbod

Terry B. Ball

VJ Jasinghe

W Huang da

PubMed

. We here aimed to define additional antiviral factors that are over-expressed in CVS from female sex workers at high risk of infection.CVS were collected from Kenyan HIV-seronegative (n = 34) and HIV-seropositive (n = 12) female sex workers, and were compared with those from HIV-seronegative low-risk women (n = 12). The highly exposed seronegative (HESN) sex workers were further divided into those with less (n = 22) or more (n = 12) than three years of documented sex work. Cationic protein-depleted CVS were assessed for HIV-neutralizing activity by a PBMC-based HIV-neutralizing assay, and then characterized by proteomics.HIV neutralizing activity was detected in all unprocessed CVS, however only CVS from the female sex worker groups maintained its HIV neutralizing activity after cationic protein-depletion. Differentially abundant proteins were identified in the cationic protein-depleted secretions including 26, 42, and 11 in the HESN>3yr, HESN<3yr, and HIV-positive groups, respectively. Gene ontology placed these proteins into functional categories including proteolysis, oxidation-reduction, and epidermal development. The proteins identified in this study include proteins previously associated with the HESN phenotype in other cohorts as well as novel proteins not yet associated with anti-HIV activities.While cationic proteins appear to contribute to the majority of the intrinsic HIV neutralizing activity in the CVS of low-risk women, a broader range of non-cationic proteins were associated with HIV neutralizing activity in HESN and HIV-positive female sex workers. These results indicate that novel protein factors found in CVS of women with high-risk sexual practices may have inherent antiviral activity, or are involved in other aspects of anti-HIV host defense, and warrant further exploration into their mode of action

Birse Kenzie D. M.

Cole Amy L.

Hirbod Taha

McKinnon Lyle

Ball Terry B.

Westmacott Garrett R.

Kimani Joshua

Plummer Frank

Cole Alexander M.

Burgener Adam

Broliden Kristina

Public Library of Science (PLOS)

Non-Cationic Proteins Are Associated with HIV Neutralizing Activity in Genital Secretions of Female Sex Workers

Cationic proteins found in cervicovaginal secretions (CVS) are known to contribute to the early antiviral immune response against HIV-infection in vitro. We here aimed to define additional antiviral factors that are over-expressed in CVS from female sex workers at high risk of infection.CVS were collected from Kenyan HIV-seronegative (n = 34) and HIV-seropositive (n = 12) female sex workers, and were compared with those from HIV-seronegative low-risk women (n = 12). The highly exposed seronegative (HESN) sex workers were further divided into those with less (n = 22) or more (n = 12) than three years of documented sex work. Cationic protein-depleted CVS were assessed for HIV-neutralizing activity by a PBMC-based HIV-neutralizing assay, and then characterized by proteomics.HIV neutralizing activity was detected in all unprocessed CVS, however only CVS from the female sex worker groups maintained its HIV neutralizing activity after cationic protein-depletion. Differentially abundant proteins were identified in the cationic protein-depleted secretions including 26, 42, and 11 in the HESN>3 yr, HESN<3 yr, and HIV-positive groups, respectively. Gene ontology placed these proteins into functional categories including proteolysis, oxidation-reduction, and epidermal development. The proteins identified in this study include proteins previously associated with the HESN phenotype in other cohorts as well as novel proteins not yet associated with anti-HIV activities.While cationic proteins appear to contribute to the majority of the intrinsic HIV neutralizing activity in the CVS of low-risk women, a broader range of non-cationic proteins were associated with HIV neutralizing activity in HESN and HIV-positive female sex workers. These results indicate that novel protein factors found in CVS of women with high-risk sexual practices may have inherent antiviral activity, or are involved in other aspects of anti-HIV host defense, and warrant further exploration into their mode of action

Kenzie D M Birse

Amy L Cole

Terry B Ball

Garrett R Westmacott

Alexander M Cole

Directory of Open Access Journals

PLoS ONE

Non-Cationic Proteins Are Associated with HIV Neutralizing Activity in Genital Secretions of Female Sex Workers.

<div>ObjectiveCationic proteins found in cervicovaginal secretions (CVS) are known to contribute to the early antiviral immune response against HIV-infection in vitro. We here aimed to define additional antiviral factors that are over-expressed in CVS from female sex workers at high risk of infection.MethodsCVS were collected from Kenyan HIV-seronegative (n = 34) and HIV-seropositive (n = 12) female sex workers, and were compared with those from HIV-seronegative low-risk women (n = 12). The highly exposed seronegative (HESN) sex workers were further divided into those with less (n = 22) or more (n = 12) than three years of documented sex work. Cationic protein-depleted CVS were assessed for HIV-neutralizing activity by a PBMC-based HIV-neutralizing assay, and then characterized by proteomics.ResultsHIV neutralizing activity was detected in all unprocessed CVS, however only CVS from the female sex worker groups maintained its HIV neutralizing activity after cationic protein-depletion. Differentially abundant proteins were identified in the cationic protein-depleted secretions including 26, 42, and 11 in the HESN>3yr, HESN<3yr, and HIV-positive groups, respectively. Gene ontology placed these proteins into functional categories including proteolysis, oxidation-reduction, and epidermal development. The proteins identified in this study include proteins previously associated with the HESN phenotype in other cohorts as well as novel proteins not yet associated with anti-HIV activities.ConclusionWhile cationic proteins appear to contribute to the majority of the intrinsic HIV neutralizing activity in the CVS of low-risk women, a broader range of non-cationic proteins were associated with HIV neutralizing activity in HESN and HIV-positive female sex workers. These results indicate that novel protein factors found in CVS of women with high-risk sexual practices may have inherent antiviral activity, or are involved in other aspects of anti-HIV host defense, and warrant further exploration into their mode of action.</div

Kenzie D. M. Birse (758108)

Amy L. Cole (119441)

Taha Hirbod (150430)

Lyle McKinnon (361117)

Terry B. Ball (302939)

Garrett R. Westmacott (485196)

Joshua Kimani (149573)

Frank Plummer (346991)

Alexander M. Cole (119444)

Adam Burgener (485195)

Kristina Broliden (60331)

The Francis Crick Institute

Crossref

Birse, Kenzie D. M.

Cole, Amy L.

Hirbod, Taha.

McKinnon, Lyle R.

Ball, Terry Blake.

Westmacott, Garrett R.

Kimani, Joshua.

Plummer, Frank.

Cole, Alexander M.

Burgener, Adam D.

Broliden, Kristina.

ResearchSpace (Univ. of KwaZulu-Natal)

Non-cationic proteins are associated with HIV neutralizing activity in genital secretions of female sex workers.

Objective: Cationic proteins found in cervicovaginal secretions (CVS) are known to contribute to the early antiviral immune response against HIV-infection in vitro. We here aimed to define additional antiviral factors that are over-expressed in CVS from female sex workers at high risk of infection.
Methods: CVS were collected from Kenyan HIV-seronegative (n=34) and HIV-seropositive (n=12) female sex workers, and were compared with those from HIV-seronegative low-risk women (n=12). The highly exposed seronegative (HESN) sex workers were further divided into those with less (n=22) or more (n=12) than three years of documented sex work. Cationic protein-depleted CVS were assessed for HIV-neutralizing activity by a PBMC-based HIV-neutralizing assay, and then characterized by proteomics.
Results: HIV neutralizing activity was detected in all unprocessed CVS, however only CVS from the female sex worker groups maintained its HIV neutralizing activity after cationic protein-depletion. Differentially abundant proteins were identified in the cationic protein-depleted secretions including 26, 42, and 11 in the HESN>3yr, HESN<3yr, and HIV-positive groups, respectively. Gene ontology placed these proteins into functional categories including proteolysis, oxidation-reduction, and epidermal development. The proteins identified in this study include proteins previously associated with the HESN phenotype in other cohorts as well as novel proteins not yet associated with anti-HIV activities.
Conclusion: While cationic proteins appear to contribute to the majority of the intrinsic HIV neutralizing activity in the CVS of low-risk women, a broader range of non-cationic proteins were associated with HIV neutralizing activity in HESN and HIV-positive female sex workers. These results indicate that novel protein factors found in CVS of women with high-risk sexual practices may have inherent antiviral activity, or are involved in other aspects of anti-HIV host defense, and warrant further exploration into their mode of action.</p

Kenzie Birse (746214)

Amanda Cole (746217)

Taha Hirbod (746218)

Lyle McKinnon (746219)

Garrett R. Westmacott (746220)

Non-cationic proteins are associated with HIV neutralizing activity in genital secretions of female sex workers

https://researchspace.ukzn.ac.za/bitstream/10413/13684/1/Birse_Kenzie_2015.pdf

Non-cationic proteins are associated with HIV neutralizing activity in genital secretions of female sex workers.

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ResearchSpace (Univ. of KwaZulu-Natal)

The Francis Crick Institute