Aydın, Ç.

Mammadov, R.

Metin, H.

Taşdelen, G.

Özay, C.

English

Электронная библиотека БГУ

Биоинженерия. Трансгенные технологии
222
HISTOLOGICAL EFFECTS OF SILYBUM MARIANUM (L.) GAERTNER
SEED EXTRACTS ON THE STOMACH MUCOSA IN RATS
Mammadov R*., Metin H., Özay C., Taşdelen G., Aydın Ç.
Pamukkale University, Denizli, Turkey; *rmammad@yahoo.com
The milk thistle Silybum marianum (L.) Gaertner, a member of the Asteraceae family,
is among the most ancient of all known herbal medicines. Various preparations of the
plant, especially the seeds (fruits), have been used medicinally for over 2000 years to treat
liver disorders. It is also referred to as holy thistle, Marian thistle, St. Mary thistle, wild
artichoke and kenger (Turkish). The seeds of milk thistle are the medicinal parts of the
plant. The primary active constituent of milk thistle is silymarin, which is composed of
four isomers: silybin, isosilybin, silychristin, and silydianin. Milk Thistle is widely used in
Europe, United States, Egypt, and elsewhere for ‘‘liver support’’. Milk thistle/silymarin is
thought to work via: (1) preventing entry of various toxins, e.g., alcohol, carbon tetrachlo-
ride and heavy metals, into hepatocytes; (2) stimulating protein synthesis with hepatocyte
regeneration; (3) acting as a free-radical scavenger and antioxidant; and (4) modulating the
immune response. Moreover, silymarin shows antidiabetic, hypolipidaemic, anti-
inflammatory, cardioprotective, neurotrophic and neuroprotective effects.
In the present study, the histological effects of Silybum marianum seed extract on stom-
ach in rats were investigated. Experiments were performed at Experimental Research Cen-
ter, Faculty of Medicine, Pamukkale University, Denizli, Turkey. The seeds of S. mari-
anum were collected in August 2011 from near Turgutlu, Manisa province (Turkey). Seeds
were air-dried, protected from direct sunlight, and then finely powdered. The powdered
seeds were put in the flask with ethanol for extraction process. Two different concentra-
tions of the extract were prepared: 1.5% and 2%. The extracts of the plant at the concentra-
tions of 1.5 and 2 ml/100 g body weight/day were administered orally to the two experi-
mental rat groups for 10 weeks. At the end of the experimental period, rats in all groups
were sacrificed by cervical dislocation. For histological experiments tissues were fixed,
processed and embedded in paraffin. 5 μm sections were stained with Hematoxylin and
Eosin, Periodic acid-Schiff stain for histomorphology.
The present work presents histological investigations in mucosal epithelium of the
stomach of mice treated with Silybum marianum seed extract. The mucous glands in stom-
ach of the experimental group revealed positive reactivities with PAS for neutral mucosub-
stances. However, mucus material at the concentration of 2% were less than the concentra-
tion of 1.5%. These results indicate that plant extract can increase mucous secretion. Pre-
sumeably, it may increase the production of glycoproteins of mucosubstances. From this
hypothesis, it can be pronounced that further investigations are needed for analysis of the
effects of the plant extract on the mucin synthesis.
Key words: Silybum marianum seeds, histology, ethanolic extract, stomach.


Histological effects of Silybum marianum (L.) gaertner seed extracts on the stomach mucosa in rats

http://elib.bsu.by/bitstream/123456789/34910/1/186.pdf

Histological effects of Silybum marianum (L.) gaertner seed extracts on the stomach mucosa in rats

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