Ashykhmina, Natallia

Flügge, Ulf-Ingo

Frerigmann, Henning

Geier, Melanie

Gigolashvili, Tamara

Haferkamp, Ilka

Kopriva, Stanislav

English

Электронная библиотека БГУ

Рост, фитогормональная регуляция, транспорт веществ,
клеточная сигнализация, функционирование цитоскелета и органелл
63
THE IMPORTANCE OF 3’-PHOSPHOADENOSINE 5’-PHOSPHOSULFATE
TRANSPORT IN THE PLANT CELL
Tamara Gigolashvili1, Natallia Ashykhmina1, Melanie Geier2, Henning Frerigmann1,
Stanislav Kopriva3, Ilka Haferkamp2 and Ulf-Ingo Flügge1
1Botanical Institute, Cologne Biocenter, University of Cologne, Cologne, Germany;
t.gigolashvili@uni-koeln.de
2Cellular Physiology / Membrane transport, Technical University of Kaiserslautern,
Kaiserslautern, Germany;
3Department of Metabolic Biology, John Innes Centre, Norwich Research Park, Nor-
wich, UK
3’-phosphoadenosine 5’-phosphosulfate (PAPS) is the high energy sulfate donor, which
is required for sulfation reactions in eucariotic cells. Plants produce PAPS mainly in plas-
tids. Accordingly, PAPS has to be provided in the cytosol to serve as substrate for sul-
fotransferase reaction sand the Golgi apparatus. Intriguingly, the corresponding PAPS
transporters in the plant cell were unknown till recently.
We were able to identify the first chloroplastidic PAPS transporter (PAPST1) in Arabi-
dopsis. Its functional characterization and the analysis of corresponding mutants demon-
strate that PAPST1 connects plastidic PAPS synthesis and cytosolic sulfation reactions. In
contrast to the known animal PAPS antiporters which are members of the nucleotide-sugar
transporter family, PAPST1 belongs to the mitochondrial carrier family.
Transport studies using the PAPST1 recombinant protein revealed that it favors PAPS,
3’-phosphoadenosine 5’-phosphate (PAP) and ATP as substrates. The protein could be de-
tected both in the plastid envelope membrane and in thylakoids and it is present in plastids
of autotrophic and heterotrophic tissues. Physiological analyses of papst1 mutant plants
additionally indicate that PAPST1 is involved in sulfur metabolism including the biosyn-
thesis of thiols, secondary metabolites and, importantly, phytosulfokines.


The importance of 3’-phosphoadenosine 5’-phosphosulfate transport in the plant cell

http://elib.bsu.by/bitstream/123456789/33481/1/027.pdf

The importance of 3’-phosphoadenosine 5’-phosphosulfate transport in the plant cell

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