Ermiş, A.

Evgen, E.

Kardeşler, A.

Çelik, A.

Özay, C.

English

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Фотосинтез, биохимические процессы, регуляция клеточного метаболизма
42
ANTIOXIDANT AND FREE RADICAL SCAVENGING ACTIVITY OF SCILLA
AUTUMNALIS BULBS AND LEAVES ETHANOLIC EXTRACTS
Özay C., Ermiş A., Evgen E., Kardeşler A., Çelik A.*
Pamukkale University, Biology Department, Denizli, Turkey; *acelik@pau.edu.tr
Our country is very rich in of plant species, tubers, onions, rhizome collected under the
name geophyt. One of the most important features if used for purpose of treatment of geo-
phyt the with the active substances they contain onions, tuber, and rhizomes. These the ac-
tive substances have antioxidant properties which to the neutralizing harmful free radicals
in body which cause many of the disease. Free radicals involved in a number of diseases
due to the oxidative damage to DNA, lipids, and proteins and which can result in failure of
cellular functions. Antioxidants have of great importance for the prevention of oxidative
stress that to cause in many illness. This reason is increasing the consumption of foods
containing antioxidants and antioxidant substances of natural origin the use of as a pre-
servative in foods. Scilla which a genus the important of between geophyta is creates a
group of natural antioxidants. Scilla is a genus of bulbous monocotyledons belonging to
the family Liliaceae. Liliaceae family is a large family which is represented with 4000 spe-
cies belonging to 280 genus in in the world. Many kinds of flavonoids, homoisoflavanones
and triterpenoid have been identified in Scilla species plant.The aim of present study was
to examine the antioxidant and phenolic content of ethanol extracts from the bulbs and
leaves from Scilla autumnalis species. Total antioxidant activity of the ethanol extracts was
evaluated by β-carotene-linoleic acid method and DPPH- free radical scavenging activity
assay. The total phenolic content of the ethanol extracts was determined using to the Folin-
Ciocalteu method. The total phenolic content of extracts was determined as gallic acid
equivalent (mg GAE/g dried sample). The highest phenolic content was found in leaves-
ethanol extract (16.3) (mg GAE/g dried sample).
Results showed that the highest antioxidant activity was in the leaves-ethanol solution
(68.7%) and the lowest antioxidant activity was in the bulbs-ethanol solution (54.3%). Ac-
cording to DPPH-free radical scavenging activity assay, the highest free radical scavenging
activity was determined in extract on leaves-ethanol (54.76%) and the least efficiency in
extract bulbs-ethanol (26.19%).
KEY WORDS: Scilla autumnalis, antioxidant, DPPH radical scavenging activity, phe-
nolic β-caroten-linoleic acid assay.


Antioxidant and free radical scavenging activity of Scilla autumnalis bulbs and leaves ethanolic extracts

http://elib.bsu.by/bitstream/123456789/33460/1/006.pdf

Antioxidant and free radical scavenging activity of Scilla autumnalis bulbs and leaves ethanolic extracts

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