Intersexuality is a rare congenital condition that leads to the development of an ambiguous urogenital tract. Removal of the reproductive tract is recommended to avoid genital diseases; however there is scant information about the procedure. The objective of this report is to describe the surgical procedure used to treat the canine intersex condition. Surgical corrections were performed on four intersex dogs: three 78 XX SRY-negative (one bilateral ovotestis, two sertolicell-only syndrome) and one 78 XX SRY-positive (sertolicell-only syndrome). Despite the different genetic and histological features, the anatomical characteristics were similar. The patients presented a vulva that lacked of dorsal fold placed ventrocranially to its anatomically normal position, a hypertrophied os clitoris protruding from the vulva and a urinary opening near to its normal position in the bitch..