Commercial dairy farming from planted pasture is commonly practised in the southern Cape region of South Africa. These pastures are established by various methods ranging in degree of disturbance from no-tillage to conventional tillage. Different establishment methods may alter the below-ground ecosystem, and depending on the degree of disturbance, lead to changes in microbial biodiversity and soil health. Disturbance of pasture soil may play an important role in regulating soil microbial community structure. The aim of this study was to investigate changes in soil microbial community-level physiological profiles of kikuyu (Pennisetum clandestinum) pastures reinforced with annual ryegrass (Lolium multiflorum) by various degrees of soil disturbance
