Clove leaf extract contains high natural antioxidant.
In this study, an experiment was conducted to analyse the
effect methanol extract of clove leaf on the profile of superoxide
dismutase (SOD) in the rabbits liver under hypercholesterolemic
condition. Rabbits were divided into three groups i.e (1) negative
control group, (2) positive control (hypercholesterolemic) group,
which fed diet containing 1% cholesterol for 50 days, and (3)
group which was given clove leaf extract and 1% cholesterol
simultaneously for 50 days. Contents profile of antioxidant
superoxide dismutase in rabbit liver tissue was obtained using
immunihistochemical techniques. The images were taken from
each group using a microscope to analyse the reaction of clove
leaf extract to the cells. The images were processed using Kmean clustering to discriminate the cells after treatment. The cell
segmentation was performed to count number of the cells based
on their treatment. The cell counting technique then compared
with the manual counting. The results demonstrate that the
developed technique was a reliable image processing technique
which could be used in the cell counting purposes
