Gavarić, Aleksandra

Vidović, Senka

Gavarić Aleksandra

Vidović Senka

University of Szeged

II. Symposium of Young Researchers on Pharmaceutical Technology, Biotechnology and Regulatory Science January 23-24th 2020. Szeged, Hungary 19  OP-14 Optimization of bioactive compounds of horehound extracts obtained using ultrasound and microwave assisted extraction: anti-hyperglycaemic activity Aleksandra Gavaric, Senka Vidovic University of Novi Sad, Faculty of Technology, Department of Biotechnology and Pharmaceutical Engineering  White horehound (Marrubium vulgare L.) is a grey-leaved perennial herb, belonging to Lamiaceae family, distributed in Eurasia and northern Africa zones [1]. According to the recent literature, horehound shows several in vivo and in vitro activities including antihypertensive, antioxidant, antiinflammatory, antidiabetic, effects on respiratory system, digestive stimulant, antiasthmatic, hypolipidemic, antibacterial and antifungal effects [2,3,4,5,6,7]. Having a scarce information about ultrasound assisted extraction (UAE) of horehound and several articles focusing on intensification of marrubiin content by microwave assisted extraction (MAE), the idea to compare these modern extraction techniques imposed. UAE and MAE were confronted in reference to extraction yield, polyphenols content, antioxidant potential and antidiabetic activity. Response surface methodology was used for optimization of process parameters in UAE and MAE. The optimal UAE parameters for maximized polyphenols and antioxidant activity were temperature of 73.6 °C, extraction time of 40 min and ultrasound power of 30.3 W/L, while in case of MAE the optimal parameters were 63.8% ethanol, extraction time of 15 min and microwave power of 422 W. The optimal UAE and MAE extracts were subjected to α-amylase and α-glucosidase inhibitory assays to determine their anti-hyperglycaemic potential.  References 1. Amri et al., Molecules, 22(11), 1851 (2017) 2. El Bardai et al., Clin. Exp. Hypertens., 26, 465–474 (2004) 3. Meyre-Silva et al., IL Farmaco, 60, 321–326 (2005) 4. Stulzer et al., J. Ethnopharmacol., 108, 379–384 (2006) 5. Elberry et al., Int. J. Diabetes Mellit., 3, 37–44 (2011) 6. Boudjelal et al., Fitoterapia, 83, 286–292 (2012) 7. Kadri et al., Afr. J. Biotechnol., 10, 3908–3914 (2011)  Supervisor: dr Senka Vidovic    DOI: 10.14232/syrptbrs.2020.op14  

Optimization of bioactive compounds of horehound extracts obtained using ultrasound and microwave assisted extraction : anti hyperglycaemic activity

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Optimization of bioactive compounds of horehound extracts obtained using ultrasound and microwave assisted extraction : anti hyperglycaemic activity

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