An efficient and novel enzymatic method was developed for the synthesis of β-aryl-fluorinecontaining β-amino acid enantiomers through lipase PS IM (Burkholderia cepasia) catalyzed hydrolysis in i-Pr2O at 45 °C in the presence of Et3N. In order to follow the enzymatic reactions, an adequate analytical method was devised for the enantioseparation of racemic βamino esters and β-amino acids
