Beatson SA

Beckham KSH

Burchmore RJ

Burgess K

Byron O

Connolly JPR

Douce GR

Gally DL

Gawthorne JA

Ritchie JM

Roe AJ

Smith BO

Tahoun A

Wang D

Wolfe AJ

Summary: Classical studies have focused on the role that individual regulators play in controlling virulence gene expression. An emerging theme, however, is that bacterial metabolism also plays a key role in this process. Our previous work identified a series of proteins that were implicated in the regulation of virulence. One of these proteins was AdhE, a bi-functional acetaldehyde-CoA dehydrogenase and alcohol dehydrogenase. Deletion of its gene (adhE) resulted in elevated levels of extracellular acetate and a stark pleiotropic phenotype: strong suppression of the Type Three Secretion System (T3SS) and overexpression of non-functional flagella. Correspondingly, the adhE mutant bound poorly to host cells and was unable to swim. Furthermore, the mutant was significantly less virulent than its parent when tested in vivo, which supports the hypothesis that attachment and motility are central to the colonization process. The molecular basis by which AdhE affects virulence gene regulation was found to be multifactorial, involving acetate-stimulated transcription of flagella expression and post-transcriptional regulation of the T3SS through Hfq. Our study reveals fascinating insights into the links between bacterial physiology, the expression of virulence genes, and the underlying molecular mechanism mechanisms by which these processes are regulated. \ua9 2014 The Authors. Molecular Microbiology published by John Wiley &amp; Sons Ltd

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The metabolic enzyme AdhE controls the virulence of <em>Escherichia coli</em> O157: H7

https://eprint.ncl.ac.uk/fulltext.aspx?url=260317/41BAAAA1-5390-4D5C-A7CE-CF00AF1587A9.pdf&pub_id=260317

The metabolic enzyme AdhE controls the virulence of <em>Escherichia coli</em> O157: H7

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