Ahmad A

Baker PJ

Batters SL

Brooker TA

Bullough PA

Dix SR

Harris MJ

Mosby DJ

Owen HJ

Rice DW

Sedelnikova SE

Shastri S

Spiewak HL

Sun R

Thomas MS

Tzokov SB

The type VI secretion system (T6SS) is a multi-protein complex that injects bacterial effector proteins into target cells. It is composed of a cell membrane complex anchored to a contractile bacteriophage tail-like apparatus consisting of a sharpened tube that is ejected by the contraction of a sheath against a baseplate. We present structural and biochemical studies on TssA subunits from two different T6SSs that reveal radically different quaternary structures in comparison to the dodecameric E. coli TssA that arise from differences in their C-terminal sequences. Despite this, the different TssAs retain equivalent interactions with other components of the complex and position their highly conserved N-terminal ImpA_N domain at the same radius from the centre of the sheath as a result of their distinct domain architectures, which includes additional spacer domains and highly mobile interdomain linkers. Together, these variations allow these distinct TssAs to perform a similar function in the complex

Newcastle University E-Prints

English

Structural insights into the function of type VI secretion system TssA subunits

https://eprint.ncl.ac.uk/fulltext.aspx?url=253147/B216DCD7-0400-4D88-A609-183D1492429B.pdf&pub_id=253147

Structural insights into the function of type VI secretion system TssA subunits

Abstract

Similar works

Full text

Available Versions

Newcastle University E-Prints