Bass I

Brodolin K

Kulbachinskiy A

Mustaev A

Severinov K

Yuzenkova Y

Zenkin N

Recognition of the -10 promoter consensus element by region 2 of the bacterial RNA polymerase  subunit is a key step in transcription initiation.  also functions as an elongation factor, inducing transcription pausing by interacting with transcribed DNA non-template strand sequences that are similar to the -10 element sequence. Here, we show that the region 1.2 of Escherichia coli 70, whose function was heretofore unknown, is strictly required for efficient recognition of the non-template strand of -10-like pause-inducing DNA sequence by  region 2, and for -dependent promoter-proximal pausing. Recognition of the fork-junction promoter DNA by RNA polymerase holoenzyme also requires  region 1.2 and thus resembles the pause-inducing sequence recognition. Our results, together with available structural data, support a model where  region 1.2 acts as a core RNA polymerase-dependent allosteric switch that modulates non-template DNA strand recognition by  region 2 during transcription initiation and elongation

Region 1.2 of the RNA polymerase sigma subunit controls recognition of the -10 promoter element

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